Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay
Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay
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DOI：
10.1038/cr.2008.38
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发表时间：
2008-04-01
期刊：
影响因子：
44.1
通讯作者：
He, Cheng
中科院分区：
文献类型：
--
作者：
Huang, Zhi-hui;Wang, Ying;He, Cheng
嗅鞘细胞(OECs)是一种独特的神经胶质细胞，具有促进轴突生长的特性。嗅鞘细胞移植已成为治疗轴索损伤和脱髓鞘疾病的一种有前景的实验性治疗方法。然而，嗅鞘细胞的一些基本细胞特性仍然不清楚。在这项研究中，我们发现基于单个分离细胞的时间推移成像，不同的OEC亚群表现出不同的迁移特性，这可能是由于它们不同的细胞骨架组织所致。此外，在单细胞迁移分析中，OEC亚群对溶血磷脂酸(LPA)的梯度表现出不同的有吸引力的迁移反应。最后，我们发现OEC亚群自发地相互转化。综上所述，这些结果首次证明，据我们所知，不同的OEC亚群在体外表现出不同的迁移特性，并为支持OEC是具有可延展功能表型的单个细胞类型的概念提供了新的证据。
Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinating diseases. However, some fundamental cellular properties of OECs remain unclear. In this study, we found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations. Moreover, OEC subpopulations displayed different attractive migratory responses to a gradient of lysophosphatidic acid (LPA) in single-cell migration assays. Finally, we found that OEC subpopulations transformed into each other spontaneously. Together, these results demonstrate, for the first time to our knowledge, that distinct OEC subpopulations display different migratory properties in vitro and provide new evidence to support the notion of OECs as a single cell type with malleable functional phenotypes.