Genome-wide analysis of core cell cycle genes in Arabidopsis
Genome-wide analysis of core cell cycle genes in Arabidopsis
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DOI：
10.1105/tpc.010445
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发表时间：
2002-04-01
期刊：
影响因子：
11.6
通讯作者：
Inzé, D
中科院分区：
文献类型：
--
作者：
Vandepoele, K;Raes, J;Inzé, D
细胞周期蛋白依赖的蛋白和细胞周期蛋白通过不同的相互作用蛋白调节真核细胞周期的进程。应用一种高质量的、基于同源性的注释协议来确定最近完成的拟南芥基因组序列中的核心细胞周期基因。总共鉴定出61个基因，属于7个选定的细胞周期调控基因家族，其中30个是现有注释的新基因或更正基因。一类新的可能的细胞周期调节因子可能是E2F/DP转录因子的竞争对手，这些转录因子介导了G1到S的进程。此外，更新了现有的拟南芥细胞周期基因命名法，并将所有基因的物理位置与基因组中分段复制的块进行了比较，结果表明22个核心细胞周期基因是通过块复制产生的。这种全基因组分析说明了植物细胞周期机制的复杂性，并为阐明未来新的家族成员的功能提供了工具。
Cyclin-dependent kinases and cyclins regulate with the help of different interacting proteins the progression through the eukaryotic cell cycle. A high-quality, homology-based annotation protocol was applied to determine the core cell cycle genes in the recently completed Arabidopsis genome sequence. In total, 61 genes were identified belonging to seven selected families of cell cycle regulators, for which 30 are new or corrections of the existing annotation. A new class of putative cell cycle regulators was found that probably are competitors of E2F/DP transcription factors, which mediate the G1-to-S progression. In addition, the existing nomenclature for cell cycle genes of Arabidopsis was updated, and the physical positions of all genes were compared with segmentally duplicated blocks in the genome, showing that 22 core cell cycle genes emerged through block duplications. This genome-wide analysis illustrates the complexity of the plant cell cycle machinery and provides a tool for elucidating the function of new family members in the future.