Ryanodine induced block of a cloned human cardiac potassium channel.
Ryanodine induced block of a cloned human cardiac potassium channel.
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Ryanodine 诱导克隆的人心脏钾通道阻断。
DOI：
10.1016/0022-2828(95)90015-2
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发表时间：
1995
影响因子：
5
通讯作者：
Seth,K
中科院分区：
文献类型：
--
作者：
Bhattacharyya,ML;Sarker,S;Seth,K
使用全细胞电压钳技术研究了兰尼定对稳定小鼠 L 细胞系中表达的克隆人心脏钾通道 (hKv1.5) 的影响。 Ryanodine 以剂量依赖性方式 (10−7−10−5M) 降低了峰值电流以及 250 ms 脉冲结束时的电流。将剂量响应数据拟合到 Hill 方程得出 IC50 为 1.2 μm。阻塞程度与电压无关。兰尼碱的峰值电流从 10−5m 处的 43.6 ± 12.5% 减少到 10−7m 处的 11.3 ± 4.8%。 Ryanodine 降低了尾电流幅度，而不改变相对于对照的时间进程。在浓度低于 10−6m 时，抑制作用仅部分可逆。
The effects of ryanodine in a cloned human cardiac potassium channel (hKv1.5) expressed in a stable mouse L cell line was studied using whole cell voltage clamp technique. Ryanodine in a dose dependent manner (10−7−10−5M) reduced the peak current as well as the current at the end of a 250 ms pulse. Dose response data fitted to a Hill equation yielded IC50of 1.2 μm. The degree of block was not voltage dependent. Peak current was reduced from 43.6 ± 12.5% at 10−5m to 11.3 ± 4.8% at 10−7m ryanodine. Ryanodine reduced the tail current amplitude without changing the time course relative to the control. The inhibition was only partially reversible at concentrations below 10−6m.