Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid
Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid
复制标题
DOI：
10.1002/jcb.22052
复制
发表时间：
2009-04
影响因子：
4
通讯作者：
Ying Liang;Qi-Fu Li;Xiuping Zhang;Song-Lin Shi;Guang-Jun Jing
中科院分区：
文献类型：
--
作者：
Ying Liang;Qi-Fu Li;Xiuping Zhang;Song-Lin Shi;Guang-Jun Jing
为了研究视黄酸（RA）诱导神经母细胞瘤SK‐N‐SH细胞分化过程中核基质蛋白（NMPs）的变化，采用免疫细胞化学方法检测分化标志物，选择性提取NMPs并进行二维凝胶电泳分析。免疫细胞化学观察显示，RA治疗后SK - N - SH细胞中神经元标记物的表达上调。同时，在SK‐N‐SH分化过程中，52个NMPs（其中41个已被鉴定）发生了显著变化；其中4个nmp通过免疫印迹进一步证实。本研究提示，神经母细胞瘤细胞的分化伴随着神经元标记物和nmp表达的改变。一些差异表达的NMPs的存在与神经母细胞瘤的增殖和分化有关。我们的研究结果可能有助于揭示NMPs与神经母细胞瘤癌变和逆转之间的关系，并阐明驱动神经细胞增殖和分化的调节原理。j .细胞。生物化学学报，26(6):849-857,2009。©2009 Wiley‐Liss, Inc。
To investigate the alteration of nuclear matrix proteins (NMPs) during the differentiation of neuroblastoma SK‐N‐SH cells induced by retinoic acid (RA), differentiation markers were detected by immunocytochemistry and NMPs were selectively extracted and subjected to two‐dimensional gel electrophoresis analysis. Immunocytochemical observation demonstrated that the expression of neuronal markers was up‐regulated in SK‐N‐SH cells following RA treatment. Meanwhile, 52 NMPs (41 of which were identified) changed significantly during SK‐N‐SH differentiation; four of these NMPs were further confirmed by immunoblotting. This study suggests that the differentiation of neuroblastoma cells was accompanied by the altered expression of neuronal markers and NMPs. The presence of some differentially expressed NMPs was related to the proliferation and differentiation of neuroblastomas. Our results may help to reveal the relationship between NMPs and neuroblastoma carcinogenesis and reversion, as well as elucidate the regulatory principals driving neural cell proliferation and differentiation. J. Cell. Biochem. 106: 849–857, 2009. © 2009 Wiley‐Liss, Inc.