A Polymer Surfactant Corona Dynamically Replaces Water in Solvent-Free Protein Liquids and Ensures Macromolecular Flexibility and Activity
A Polymer Surfactant Corona Dynamically Replaces Water in Solvent-Free Protein Liquids and Ensures Macromolecular Flexibility and Activity
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DOI：
10.1021/ja303894g
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发表时间：
2012-08-15
影响因子：
15
通讯作者：
Weik, Martin
中科院分区：
文献类型：
--
作者：
Gallat, Francois-Xavier;Brogan, Alex P. S.;Weik, Martin
无溶剂蛋白质-聚合物表面活性剂杂化物中生物活性的观察挑战了水性和非水性溶剂是与功能相关的蛋白质动力学的独特促进剂的观点。在这里，我们结合联合收割机弹性非相干中子散射和特定的氘标记，分别研究蛋白质和聚合物的运动在无溶剂的杂交。发现杂交体内的肌红蛋白运动与水合蛋白质的运动非常相似，并且聚合物表面活性剂涂层的运动与水合水的运动相似，从而得出结论：聚合物表面活性剂涂层以类似于水合水的方式增塑蛋白质结构。
The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.