Effects of sulphamethazine and zinc on the functional diversity of microbial communities during composting
Effects of sulphamethazine and zinc on the functional diversity of microbial communities during composting
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DOI：
10.1080/09593330.2015.1115131
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发表时间：
2016-06-02
影响因子：
2.8
通讯作者：
Wang, Xiaojuan
中科院分区：
文献类型：
--
作者：
Duan, Manli;Yang, Jiu;Wang, Xiaojuan
用Biolog法研究了堆肥过程中锌(锌，0,600,1800 mg/kg)和磺胺二甲嘧啶(SM2，0，1，25 mg/kg)单一或复合添加对堆肥基质中微生物群落功能多样性的影响。在堆肥的第6天(高温期)和第25天(成熟期)从猪粪和小麦秸秆组成的堆肥基质中提取微生物群落。结果表明，在第6天，随着SM2和锌浓度的增加，香农指数、平均色泽展开率和基质利用率显著降低。SM2和锌联合添加的负面影响小于单独添加SM2和锌的影响。在第25天，抑制作用消失，微生物代谢活性高于第6天。通过主成分分析和聚类分析，可以进一步区分SM2和锌的作用。第6天，按PC1和PC2分为三组。在第25天，PCA样地中不同处理的分离变得越来越明显。综上所述，SM2和锌对堆肥过程中微生物群落的影响在高温期最为明显，微生物活性在成熟期恢复。在单独添加添加剂的情况下，Sm2和Zn的组合抑制作用减弱。
The changes in the functional diversity of the microbial community in a compost matrix with a single or compound addition of zinc (Zn; 0, 600, and 1800 mg/kg) and sulphamethazine (SM2; 0, 1, and 25 mg/kg) were studied with the Biolog method during composting. The microbial community was extracted from the compost matrix comprising swine manure and wheat straw at day 6 (themophilic period) and day 25 (mature period) of composting. Results proved that the Shannon index, average well-colour development, and substrate utilization significantly decreased as the concentrations of SM2 and Zn increased on day 6. The negative effect of the combined addition of SM2 and Zn was lower than that of the individual addition of SM2 and Zn. On day 25, the inhibition effect disappeared, and microbial metabolic activities were higher than those on day 6. The effects of SM2 and Zn could be further differentiated via the principal component analysis (PCA) and cluster analysis. On day 6, the treatments were divided into three groups by PC1 and PC2. The separation of the different treatments in the PCA plots became increasingly apparent on day 25. In conclusion, the effects of SM2 and Zn on the microbial community during composting became evident in the themophilic period and that the microbial activity recovered in the mature period. The combination of SM2 and Zn decreased the inhibition with the addition of individual additive.