INFLUENCES OF EXTRACELLULAR-MATRIX COMPONENTS ON THE GROWTH AND DIFFERENTIATION OF RUMINAL EPITHELIAL-CELLS IN PRIMARY CULTURE
INFLUENCES OF EXTRACELLULAR-MATRIX COMPONENTS ON THE GROWTH AND DIFFERENTIATION OF RUMINAL EPITHELIAL-CELLS IN PRIMARY CULTURE
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DOI：
10.1016/0034-5288(93)90018-b
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发表时间：
1993-01-01
影响因子：
2.4
通讯作者：
MARTENS, H
中科院分区：
文献类型：
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作者：
GALFI, P;MARTENS, H
采用绵羊瘤胃上皮细胞原代培养方法，研究了Ⅰ型和Ⅳ型胶原蛋白以及添加不同激素和丁酸钠的培养液对细胞形态和生长特性的影响。I型和IV型胶原蛋白均导致细胞增殖增加，其中刺激作用在IV型胶原蛋白中更明显。在胶原蛋白I上生长的培养物中，发现非分层和分层的菌落，而胶原蛋白IV上生长的培养物显示出主要的分层生长。分层和非分层集落中的细胞角蛋白抗体阳性。在未分层的集落中，在细胞上方和周围的网络中发现了纤维连接蛋白抗体（FN-15）的阳性染色。这表明，非复层瘤胃上皮细胞在某些方面类似于“非分化角质形成细胞”株，来自新生儿包皮表皮。分层集落中的细胞结合荆豆凝集素（UEAI），该凝集素已被证明对瘤胃粘膜中的分化上皮细胞具有特异性。补充培养基中的胰高血糖素和胰岛素增加了总细胞过度生长的面积胶原蛋白I文化，而这种影响是不存在的文化中生长的胶原蛋白IV。在两种培养物上生长的胶原蛋白I或IV，氢化可的松导致总细胞过度生长的面积增加，而钠-正丁酸盐抑制增殖。
Primary cultures of ovine ruminal epithelial cells were made to study the influence of collagen types I and IV and of medium supplementation with various hormones and Na-n-butyrate on cell morphology and growth characteristics. Both collagen type I and type IV led to increased cell proliferation with the stimulatory effect being more pronounced in collagen IV. In cultures grown on collagen I, both non-stratified and stratified colonies were found, whereas cultures grown on collagen IV showed predominantly stratified growth. Cells in both stratified and non-stratified colonies were positive for cytokeratin antibody. In non-stratified colonies, positive staining with fibronectin antibodies (FN-15) was found in a network over and around the cells. It is suggested that the non-stratified ruminal epithelial cells are in some respects similar to a 'non-differentiating keratinocyte' strain, derived from newborn foreskin epidermis. Cells in stratified colonies bound Ulex europaeus (UEAI) lectin which has been shown to be specific for differentiated epithelial cells in ruminal mucosa. Supplementation of culture medium with glucagon and insulin increased the total cell-overgrown area of collagen I cultures, whereas this effect was absent in cultures grown on collagen IV. In both cultures grown on collagen I or IV, hydrocortisone led to an increase in total cell-overgrown area, whereas Na-n-butyrate inhibited proliferation.