Gene expression profiling analysis of Mycobacterium tuberculosis genes in response to salicylate
Gene expression profiling analysis of Mycobacterium tuberculosis genes in response to salicylate
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DOI：
10.1007/s00203-005-0037-9
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发表时间：
2005-11-01
影响因子：
2.8
通讯作者：
Zhang, Y
中科院分区：
文献类型：
--
作者：
Denkin, S;Byrne, S;Zhang, Y
水杨酸盐刺激结核分枝杆菌的耗氧并诱导其产生多重耐药。为了深入了解上述观察结果中涉及的机制，对M.结核病基因对水杨酸盐的反应。水杨酸盐，除了高度诱导27 kD基因（Rv 0560 c），以前确定为高度水杨酸诱导，也引起了一系列基因的转录增加，包括一个开放的阅读框（Rv 0559 c），位于27 kD基因的下游，和一些膜/跨膜蛋白，可能作为潜在的外排泵或孔蛋白。水杨酸盐还通过下调参与RNA和蛋白质合成以及ATP合成的一系列基因而导致转录和翻译以及能量产生的普遍关闭。本文还讨论了水杨酸盐调控基因在水杨酸盐诱导的结核杆菌耐药中的作用以及水杨酸盐刺激结核杆菌耗氧的独特作用。
Salicylate stimulates the oxygen consumption and also induces multiple drug resistance in Mycobacterium tuberculosis. To gain insight into the mechanisms involved in the above observations, a microarray analysis of M. tuberculosis genes in response to salicylate was performed. Salicylate, besides highly inducing the 27 kD gene (Rv0560c) previously identified as highly salicylate-inducible, also caused increased transcription of a range of genes including an open reading frame (Rv0559c) that is located immediately downstream of the 27 kD gene, and some membrane/transmembrane proteins that may serve as potential efflux pumps or porins. Salicylate also caused a general shutdown of transcription and translation and energy production by down-regulating a range of genes involved in RNA and protein synthesis and ATP synthesis. The role of the salicylate-regulated genes in salicylate induced drug resistance and its unique effect on stimulating oxygen consumption in tubercle bacillus is discussed.