p56lck activity and expression in peripheral blood lymphocytes from patients with systemic lupus erythematosus
p56lck activity and expression in peripheral blood lymphocytes from patients with systemic lupus erythematosus
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DOI：
10.3109/08916939908995380
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发表时间：
1999-01-01
期刊：
影响因子：
3.5
通讯作者：
Szegli, G
中科院分区：
文献类型：
--
作者：
Matache, C;Stefanescu, M;Szegli, G
本研究分析了系统性红斑狼疮（SLE）患者和健康人外周血淋巴细胞（PBL）p56（lck）蛋白酪氨酸激酶的活性和表达。用非放射性酪氨酸激酶测定试剂盒测定的p56（lck）活性在活动性SLE PBL中显著较高，并将这组患者与非活动性SLE患者（p = 0.002）和健康供体（p = 0.009）区分开来。与健康供体相比，SLE淋巴细胞中的p56（lck）水平降低（尤其是对于非活动性SLE淋巴细胞，p = 0.005）。活动期SLE淋巴细胞p56（lck）的比活性明显高于非活动期SLE（p = 0.022）或健康供体淋巴细胞（p = 0.006）。活动期SLE淋巴细胞p56（lck）活性与酪氨酸磷酸化水平呈正相关。
In this study we analyzed the activity and the expression of p56(lck) protein tyrosine kinase in peripheral blood lymphocytes (PBLs) from systemic lupus erythematosus (SLE) patients and from healthy donors. The p56(lck) activity, determined by a non-radioactive Tyrosine Kinase Assay Kit, was significantly higher in active SLE PBLs and discriminated this group of patients from inactive SLE patients (p = 0.002) and healthy donors (p = 0.009), p56(lck) level decreased in SLE lymphocytes (especially for inactive SLE lymphocytes, p = 0.005) when compared to healthy donors. These differences were also reflected by the specific activity of p56(lck) that was clearly elevated in active SLE lymphocytes when compared to inactive SLE (p = 0.022) or healthy donors lymphocytes (p = 0.006), A positive correlation between the activity of p56(lck) and the tyrosine phosphorylation level in active SLE lymphocytes was found.