Dengue virus infection rate in field populations of female Aedes aegypti and Aedes albopictus in Singapore
Dengue virus infection rate in field populations of female Aedes aegypti and Aedes albopictus in Singapore
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DOI：
10.1046/j.1365-3156.2002.00873.x
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发表时间：
2002-04-01
影响因子：
3.3
通讯作者：
Pang, FY
中科院分区：
文献类型：
--
作者：
Chung, YK;Pang, FY
我们开发了一个单步逆转录-聚合酶链反应（RT-PCR），然后使用一个上游的共识引物和四个类型特异性引物内的非结构蛋白基因（NS 3）的登革病毒分型的女性伊蚊野外种群的登革病毒的巢式PCR。这分别产生了1、2、3和4型登革病毒的169、362、265和426个碱基对的诊断片段。从1997年到2000年，781只埃及伊蚊和2256只白纹伊蚊中分别有54只（6.9%）和67只对登革病毒呈阳性反应，呈下降趋势。在伊蚊中检测到的最常见的登革热病毒类型是登革热-1。详细的变化，一个血清型到另一个在蚊子种群连续三年进行了讨论。
We developed a single-step reverse transcription-polymerase chain reaction (RT-PCR) followed by a seminested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein gene (NS3) of dengue viruses to type dengue viruses in field populations of female Aedes mosquitoes. This yielded diagnostic fragments of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3 and 4, respectively. From 1997 to 2000, 54 (6.9%) of 781 Aedes aegypti and 67 of 2256 Aedes albopictus screened were positive for dengue viruses, with a declining trend. The most common dengue virus type detected in the Aedes mosquitoes was dengue-1. Details on the change of one serotype to another in the mosquito population over three consecutive years are discussed.