Designing and in Silico Analysis of PorB Protein from Chlamydia Trachomatis for Developing a Vaccine Candidate
Designing and in Silico Analysis of PorB Protein from Chlamydia Trachomatis for Developing a Vaccine Candidate
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DOI：
10.1055/s-0042-110319
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发表时间：
2016-09-01
期刊：
影响因子：
2.2
通讯作者：
Bahador, A.
中科院分区：
文献类型：
--
作者：
Pourhajibagher, M.;Bahador, A.
巴克兰：沙眼衣原体是一种专性的，细胞内的，革兰氏阴性细菌，导致性传播感染。外膜蛋白PorB是一种保守的衣原体蛋白，其功能为孔蛋白，并且是中和抗体（Abs）的靶点;因此，使其对于疫苗开发非常重要。沙眼衣原体，并解释了它的特点与生物信息学工具的帮助下，介绍它作为一个候选人的新药和疫苗设计。在这项研究中，理化特性，二级和三维结构，和功能位点预测进行了研究。结果：PorB是一种由340个氨基酸组成的表面暴露蛋白，其主要结构为无规卷曲结构（61.76%）。PorB存在于细胞外，预测表位的最大长度为氨基酸91-108，即，18个氨基酸长。该表位可用于设计抗C.结论：尽管人们在研制沙眼衣原体疫苗方面做了许多努力，但在临床上，沙眼衣原体的感染率仍很低。沙眼，迄今没有保护性疫苗。应针对PorB进行更详细的研究，以设计针对C的疫苗。由于存在不同的免疫方案和需要不同的保护机制，沙眼。
Bakground: Chlamydia trachomatis is an obligate, intracellular, gram-negative bacterium that causes sexually transmitted infections. The outer membrane protein PorB is a conserved chlamydial protein that functions as a porin and is a target for neutralizing antibodies (Abs); thus, making it important for vaccine development.Methods: We used an in silico strategy and homology modeling algorithms and focused on PorB of C. trachomatis and explained its characterization with the help of bioinformatic tools to introduce it as a candidate for novel drug and vaccine design. In this study, physicochemical characterization, secondary and 3D structure, and functional site prediction were investigated. Then, a B cell epitope was analyzed using Immune Epitope Database, which predicts the target region and helps in vaccine development.Results: PorB is a surface-exposed protein comprising 340 amino acids and frequently appears (61.76 %) as a random coiled structure. PorB was present outside the cell and the maximum length of the predicted epitope was from amino acids 91-108, i.e., 18 amino acids long. This epitope can be considered for designing Abs and vaccines against C. trachomatis.Conclusion: Although many attempts have been made to develop a vaccine against C. trachomatis, no protective vaccines are available to date. More detailed studies focusing on PorB should be performed to design vaccines against C. trachomatis because of the presence of different immunization protocols and requirement of different protective mechanisms.