Comparative histology and vibration of the vocal folds: Implications for experimental studies in microlaryngeal surgery
Comparative histology and vibration of the vocal folds: Implications for experimental studies in microlaryngeal surgery
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DOI：
10.1097/00005537-200005000-00011
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发表时间：
2000-05-01
期刊：
影响因子：
2.6
通讯作者：
Reinisch, L
中科院分区：
文献类型：
--
作者：
Garrett, CG;Coleman, JR;Reinisch, L
目标/假设：通过对不同种属动物声带显微瓣手术平面和喉动态喉镜（LVS）的组织学比较，确定最适合声带手术和功能实验研究的动物模型。第二个目标是确定人类和三种不同动物种属的分层声带结构如何影响固有层研究设计前瞻性实验室内的手术解剖。方法：将犬、猴和猪的三个喉与三个离体人喉进行比较。在每个喉的一个声带上进行微瓣手术。使用弹性蛋白、成熟胶原和基质特异性染色对手术和非手术声带进行组织学检查。根据组织学结果，在第一只宠物形成气管切开术以通过声门通气和气流后，对两只狗和两只猪进行LVS。结果：胶原和弹性蛋白纤维的分布被发现不同的物种之间的浓度不同的物种。与人类声带不同，人类声带在固有层的深层具有较高的弹性蛋白浓度，猪和狗都有一条薄的弹性蛋白带集中在浅层的基底膜区。就在这条细带的深处，胶原蛋白和弹性蛋白的浓度较低。猴子的声带有一个非常薄的粘膜层，整个粘膜中的弹性蛋白较少。犬、猪和人声带中的微瓣解剖是相似的，位于弹性蛋白和成熟胶原蛋白不太集中的浅层固有层部分内。声带微瓣平面位于声带纤维附近较深。尽管在弹性蛋白浓度的差异，在狗和猪的微皮瓣平面被发现是类似于在人类。狗的解剖结构更适合于微支撑喉镜和频闪镜检查。狗声带以与人类声带相似的方式振动，具有粘膜波和垂直相位差，这些特征在猪声带中未见。结论：基于组织学和频闪结果，认为犬是声带手术研究的更合适动物模型，因为没有动物的喉部解剖结构与人类相同。所示的犬LVS模型允许进行纵向喉部研究，需要在多个时间段重复检查，并在处死时应用组织学相关性。
Objectives/Hypothesis: To determine the most suitable animal model for experimental studies on vocal fold surgery and function by a histological comparison of the microflap surgical plane and laryngeal videostroboscopy (LVS) in different species of animals. A second goal was to determine how the layered vocal fold structure in humans and three different animal species affects surgical dissection within the lamina propria Study Design Prospective laboratory. Methods: Three larynges each from dogs, monkeys, and pigs were compared with three ex vivo human larynges. Microflap surgery was performed on one vocal fold from each larynx. Both the operated and nonoperated vocal folds were examined histologically using stains specific for elastin, mature collagen, and ground substance. Based on the histological results, LVS was performed on two dogs and two pigs after first pet-forming a tracheotomy for ventilation and airflow through the glottis. Arytenoid adduction sutures were placed to facilitate vocal fold adduction Results: The distributions of the collagen and elastin fibers were found to differ among the species with concentrations varying within species. Unlike the human vocal fold, which has a higher elastin concentration in the deeper layers of the lamina propria, both the pig and the dog had a thin band of elastin concentrated just deep to the basement membrane zone in the superficial layer. Just deep to this thin band, the collagen and the elastin were less concentrated The monkey vocal fold had a very thin mucosal layer with less elastin throughout the mucosa. The microflap dissections in each of the dog, pig, and human vocal folds were similar, being located within that portion of the superficial lamina propria where the elastin and mature collagen are less concentrated. The microflap plane in the monkey vocal fold was more deeply located near the vocalis fibers. Despite the differences in elastin concentration, the microflap plane in both the dog and the pig was found to be similar to that in humans. The dog anatomy was much more suitable for microsuspension laryngoscopy and stroboscopic examination The dog vocal folds vibrated in a similar fashion to human vocal folds with mucosal waves and vertical phase differences, features not seen in the pig vocal folds. Conclusions: Based on both the histological and stroboscopic results, the dog was believed to be a more suitable animal model for studies on vocal fold surgery, acknowledging that no animal's laryngeal anatomy is identical to that of the human. The dog LVS model presented allows for longitudinal laryngeal studies requiring repeated examinations at multiple time periods with histological correlation applied at sacrifice.