Changes in tumor‐specific antigen expression during passage in vitro and in vivo of newly derived methylcholanthrene‐induced sarcomas of BALB/c mice
Changes in tumor‐specific antigen expression during passage in vitro and in vivo of newly derived methylcholanthrene‐induced sarcomas of BALB/c mice
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新衍生的甲基胆蒽诱导的 BALB/c 小鼠肉瘤在体外和体内传代过程中肿瘤特异性抗原表达的变化
DOI：
10.1002/ijc.2910250213
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发表时间：
1980
影响因子：
6.4
通讯作者：
L. Law
中科院分区：
文献类型：
--
作者：
L. Law
在一系列甲基胆蒽诱导的纯种BALB/cAN小鼠肉瘤中，研究了移植型肿瘤抗原TATA的特征。当在同基因宿主中检测肿瘤排斥反应时，6种肉瘤中的每一种都表现出独特的TATA。然而，在三例CI‐1、CII‐5和CII‐7肉瘤中，TATA活性在早期体内传代过程中丢失。这种活性在体内从CI‐1和CII‐7的体外传代细胞系中重新建立，这些肉瘤与CI‐3、CI‐4和CII‐10一起在50个移植传代中保持稳定的TATA表型；然而，在CII‐5中，TATA永久性地消失了。这些结果表明早期传代肿瘤具有二态性。在这些肉瘤中未检测到交叉反应抗原，包括甲氧甲胺素A，一种以膜抗原为特征的肉瘤；在具有多个单倍型的BALB/c F1杂交株中进行肿瘤排斥试验和H‐2血清学研究，也没有获得任何证据表明存在外来（不适当的）H‐2抗原。在三种肉瘤中，发现（BALB/cXDBA/2）F1杂交体不能被各自的肉瘤免疫，从而获得了非H - 2型异体抗原存在的一些证据。这些发现提示在这些肉瘤中存在一种在DBA/2小鼠中正常表达的肿瘤抗原，尽管尚未获得明确的证据。MuLV、病毒结构蛋白gp70和p30以及逆转录酶的检测结果显示，3个肉瘤呈阳性，另外3个肉瘤以及甲氧甲胺A呈阴性。MuLV及其抗原对肿瘤排斥活性没有影响，放射性同位素足垫试验（IFP）中观察到的交叉反应性也与MuLV无关。
The characteristics of tumor antigens of the transplantation type, TATA, were studied in a series of methylcholanthrene‐induced sarcomas in pedigreed BALB/cAN mice. Each of the six sarcomas exhibited unique TATA when assayed for tumor rejection in syngeneic hosts. In three instances, however, in sarcomas CI‐1, CII‐5 and CII‐7, TATA activity was lost during early in vivo passages. This activity was reestablished in vivo from the in vitro‐passaged lines in CI‐1 and CII‐7 and these sarcomas along with CI‐3, CI‐4 and CII‐10 maintained a stable TATA phenotype throughout the 50 transplant passages; TATA was lost permanently, however, in CII‐5. These results indicate a dimorphic nature of early‐passage neoplasms. No crossreacting antigens were detected among these sarcomas, including also Meth A, a sarcoma well characterized as to its membrane antigens; nor was any evidence obtained for the existence of alien (inappropriate) H‐2 antigens employing tumor rejection assays in F1 hybrids of BALB/c with strains bearing several haplotypes and also with studies of H‐2 serology. In three sarcomas, some evidence was obtained for the existence of alloantigens of the non‐H‐2 type since it was found that (BALB/cXDBA/2)F1 hybrids could not be immunized by the respective sarcomas. These findings suggest the existence on these sarcomas of a tumor antigen that is expressed normally in DBA/2 mice, although no definitive evidence has been otained. Assays for MuLV, for the viral structural proteins gp70 and p30 and for reverse transcriptase showed that three sarcomas were positive and three others, as well as Meth A, were negative. MuLV and its antigens had no influence on tumor rejection activity nor could the cross‐reactivity observed in the radioisotopic footpad assay (IFP) be related to MuLV.