Physicochemical, rheological and thermal properties of Mesona chinensis polysaccharides obtained by sodium carbonate assisted and cellulase assisted extraction
Physicochemical, rheological and thermal properties of Mesona chinensis polysaccharides obtained by sodium carbonate assisted and cellulase assisted extraction
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碳酸钠辅助和纤维素酶辅助提取凉粉草多糖的理化、流变和热性质
DOI：
10.1016/j.ijbiomac.2018.12.211
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发表时间：
2019
影响因子：
8.2
通讯作者：
Xie Jianhua
中科院分区：
文献类型：
--
作者：
Xiao Yuehuan;Liu Suchen;Shen Mingyue;Jiang Lian;Ren Yanming;Luo Yu;Wen Huiliang;Xie Jianhua
凉粉草作为中国民间药材，在亚洲已有数百年的历史，是制作“黑豆凉粉”的主要原料。采用纤维素酶辅助提取法（CAE）和碳酸钠辅助提取法（SAE）分别提取凉粉草中的两种多糖（MCP-C和MCP-S）。采用离子色谱、高效凝胶渗透色谱、傅里叶变换红外光谱、扫描电镜、流变学和热重分析等方法对两种多糖的不同理化性质、流变学和热性质进行了分析。结果表明，MCP-C的总糖和糖醛酸含量高于MCP-S，而蛋白质含量和相对分子质量低于MCP-S。扫描电镜观察表明，MCP-S和MCP-C的表面形貌不同，但均具有典型的红外光谱特征和多糖的无定形形貌。流变实验表明，MCP-C和MCP-S具有典型的假塑性流体行为。MCP-S的表观粘度（η）、储能模量（G′）和复数粘度（η*）均高于MCP-C。在MCP-C和MCP-S中观察到三阶段分解模式。MCP-S比MCP-C更稳定，具有更高的起始分解温度（Ti）。
Mesona chinensishas been used as a Chinese folk medicine and main ingredient used to make “black bean jelly” in Asia for hundreds of years. In this study, two polysaccharides (MCP-C and MCP-S) fromMesona chinensiswere extracted by using cellulase assisted extraction (CAE) and sodium carbonate assisted extraction (SAE), separately. Then the different physicochemical characteristics, rheological and thermal properties of two polysaccharides were analyzed by ion chromatography, high-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rheological and thermogravimetric analysis. Results indicated that the contents of total sugar and uronic acid of MCP-C were higher than MCP-S, while the protein content and molecular weight was lower than that of MCP-S. MCP-S and MCP-C had differences in the surface morphology by SEM, while they all had the typical IR spectra characteristic and amorphous morphology of polysaccharides. The rheological experiments showed that MCP-C and MCP-S exhibited typical pseudoplasticity fluids behavior. The apparent viscosity (η), storage modulus (G′) and complex viscosity (η*) of MCP-S were higher than MCP-C. The three-stage decomposition patterns were observed in MCP-C and MCP-S. MCP-S was more stable and had higher initial decomposition temperature (Ti) than MCP-C.