Microarray analysis of differential gene expression elicited in Trametes versicolor during interspecific mycelial interactions
Microarray analysis of differential gene expression elicited in Trametes versicolor during interspecific mycelial interactions
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DOI：
10.1016/j.funbio.2010.05.006
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发表时间：
2010-08-01
期刊：
影响因子：
2.5
通讯作者：
Rogers, Hilary J.
中科院分区：
文献类型：
--
作者：
Eyre, Catherine;Muftah, Wafa;Rogers, Hilary J.
杂色栓菌是一种重要的白色腐朽菌，具有重要的工业和生态价值。腐解型担子菌是林地生态系统中的主要分解菌，很少形成单种种群，因此种间菌丝相互作用频繁发生。相互作用有不同的结果，包括一个物种被另一个物种取代或僵局。我们已经建立了消减cDNA文库来富集T. versicolor与另一种腐食性担子菌Gausapatum相互作用，这种相互作用导致后者的取代。表达序列标签（EST）（1920）用于微阵列分析，并比较它们在与三种不同真菌相互作用期间的表达：S。gausapatum（由T. versicolor）、Bjerkandera adusta（deadlock）和Hypholoma fasciculare（替代T. versicolor）。在T. versicolor和S. gausapatum或B。adusta与H.束，表明相互作用的结果和基因表达的变化之间的关系。(C)2010年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Trametes versicolor is an important white rot fungus of both industrial and ecological interest. Saprotrophic basidiomycetes are the major decomposition agents in woodland ecosystems, and rarely form monospecific populations, therefore interspecific mycelial interactions continually occur. Interactions have different outcomes including replacement of one species by the other or deadlock. We have made subtractive cDNA libraries to enrich for genes that are expressed when T. versicolor interacts with another saprotrophic basidiomycete, Stereum gausapatum, an interaction that results in the replacement of the latter. Expressed sequence tags (ESTs) (1920) were used for microarray analysis, and their expression compared during interaction with three different fungi: S. gausapatum (replaced by T. versicolor), Bjerkandera adusta (deadlock) and Hypholoma fasciculare (replaced T. versicolor). Expression of significantly more probes changed in the interaction between T. versicolor and S. gausapatum or B. adusta compared to H. fasciculare, suggesting a relationship between interaction outcome and changes in gene expression. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.