Preparation of comb-type N-isopropylacrylamide hydrogel beads and their application for size-selective separation media
Preparation of comb-type N-isopropylacrylamide hydrogel beads and their application for size-selective separation media
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DOI：
10.1021/bm025697q
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发表时间：
2003-03-01
影响因子：
6.2
通讯作者：
Okano, T
中科院分区：
文献类型：
--
作者：
Annaka, M;Matsuura, T;Okano, T
采用反相悬浮聚合法制备了一系列梳型聚N-异丙基丙烯酰胺（NIPAM）凝胶球。梳型NIPAM凝胶珠在30 ℃下表现出大的体积变化，并且它们的去溶胀速率（定义为半收缩所需的时间）比普通型NIPAM凝胶珠快10倍。凝胶珠用于浓缩白蛋白、γ-球蛋白和维生素B-12的稀水溶液。梳型NIPAM凝胶对白蛋白和丙种球蛋白的分离效率分别为80%和85%。而正常型NIPAM凝胶分别为55%和60%。将接枝链并入凝胶中使得有效筛目尺寸更小。因此，它诱导了溶质和网络之间的额外阻塞效应，并排除了高分子量溶质。在它们提取了水之后，它们的快速去溶胀特性使得凝胶通过加热释放吸收的水而有效地再生。
A series of the comb-type poly(N-isopropylacrylamide) (NIPAM) gel beads were prepared by inverse suspension polymerization techniques. The comb-type NIPAM gel beads exhibited large volume change at 30 degreesC, and their deswelling rate, defined as the time required for half-shrinking, was 10 times faster than that of the normal-type NIPAM gel beads. The gel beads were utilized to concentrate dilute aqueous solutions of albumin, gamma-globulin, and vitamin B-12. The separation efficiencies of albumin and gamma-globulin with the comb-type NIPAM gel were 80% and 85%, respectively. Whereas those with normal-type NIPAM gel were 55% and 60%, respectively. The incorporation of grafted chains into gel makes the effective mesh size smaller. Therefore it induces the additional obstruction effects between the solutes and network and excludes the high molecular weight solutes. After they have extracted water, their rapid deswelling property makes the gel regenerate effectively by warming to release the absorbed water.