Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink
Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink
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曼氏乌贼墨汁硫酸化多糖的抗转移和抗血管生成活性
DOI：
10.1016/j.carbpol.2012.08.050
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发表时间：
2013-01-02
影响因子：
11.2
通讯作者：
Wang, Fengshan
中科院分区：
文献类型：
--
作者：
Zong, Aizhen;Zhao, Ting;Wang, Fengshan
先前的研究表明，SIP-SII（一种硫酸化乌贼墨汁多糖）通过抑制基质金属蛋白酶-2（MMP-2）的蛋白水解活性来抑制癌细胞的侵袭和迁移。因此，本研究探讨了SIP-SII的体内抗转移作用。 SIP-SII（15 和 30 mg/kg·d）使小鼠模型中的 B16F10 肺转移率分别显着降低 85.9% 和 88.0%。免疫组织化学显示，SIP-SII 降低肺转移结节中细胞间粘附分子 1 (ICAM-1) 和碱性成纤维细胞生长因子 (bFGF) 的表达。此外，在鸡绒毛尿囊膜测定中，0.08-2 mg/mL 的 SIP-SII 抑制新生血管形成。在体外实验中，SIP-SII（0.8-500μg/mL）分别显着降低SKOV3和EA.hy926细胞中ICAM-1和bFGF的蛋白和mRNA表达。这些结果表明SIP-SII可能通过抑制ICAM-1介导的肿瘤粘附和bFGF介导的血管生成来抑制黑色素瘤转移，并抑制癌细胞的侵袭和迁移。 (C) 2012 Elsevier Ltd. 保留所有权利。
A previous study demonstrated that SIP-SII, a sulfated Sepiella maindroni ink polysaccharide, suppressed the invasion and migration of cancer cells via the inhibition of the proteolytic activity of matrix metalloproteinase-2 (MMP-2). Therefore, this study investigated the anti-metastatic effect of SIP-SII in vivo. SIP-SII (15 and 30 mg/kg d) markedly decreased B16F10 pulmonary metastasis in mice models by 85.9% and 88.0%, respectively. Immunohistochemistry showed that SIP-SII decreased the expression of the intercellular adhesion molecule 1 (ICAM-1) and basic fibroblast growth factor (bFGF) in lung metastasis nodules. In addition, SIP-SII inhibited neovascularization in chick chorioallantoic membrane assay at 0.08-2 mg/mL. In the in vitro experiments, SIP-SII (0.8-500 mu g/mL) significantly decreased the protein and mRNA expression of ICAM-1 and bFGF in SKOV3 and EA.hy926 cells, respectively. These results suggested that SIP-SII might suppress melanoma metastasis via the inhibition of the tumor adhesion mediated by ICAM-1 and the angiogenesis mediated by bFGF, as well as resulting in depression of the invasion and migration of carcinoma cells. (C) 2012 Elsevier Ltd. All rights reserved.