HUMAN CELL-SURFACE GLYCOPROTEIN RELATED TO CELL-PROLIFERATION IS THE RECEPTOR FOR TRANSFERRIN
HUMAN CELL-SURFACE GLYCOPROTEIN RELATED TO CELL-PROLIFERATION IS THE RECEPTOR FOR TRANSFERRIN
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DOI：
10.1073/pnas.78.5.3039
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
OMARY, MB
中科院分区：
文献类型：
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作者：
TROWBRIDGE, IS;OMARY, MB
通过在单克隆抗体-琼脂糖柱上的亲和色谱法，从培养的人白血病胸腺衍生（T）细胞系的脱氧胆酸盐溶解膜中纯化在增殖细胞上选择性表达的表观MW约为100，000的细胞表面糖蛋白抗原。通过用亲和纯化的糖蛋白免疫制备的常规异种抗血清被发现含有针对与培养细胞紧密结合的血清组分的抗体。该分子与通过免疫沉淀从细胞裂解物中纯化的细胞表面糖蛋白特异性相关。血清成分经鉴定为转铁蛋白。显然，膜糖蛋白是细胞表面转铁蛋白受体。
A cell surface glycoprotein antigen with an apparent MW of about 100,000 that is selectively expressed on proliferating cells was purified from deoxycholate-solubilized membranes of a cultured human leukemic thymus-derived (T) cell line by affinity chromatography on a monoclonal antibody-Sepharose column. A conventional xenoantiserum prepared by immunization with the affinity-purified glycoprotein was found to contain antibodies against a serum component that bound tightly to cultured cells. This molecule was specifically associated with the cell surface glycoprotein purified by immunoprecipitation from lysates of cells. The serum component was identified as transferrin. Apparently, the membrane glycoprotein is the cell surface transferrin receptor.