Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation
Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation
复制标题
三维石墨烯通过代谢调节增强神经干细胞增殖
DOI：
10.3389/fbioe.2019.00436
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发表时间：
2020-01-08
影响因子：
5.7
通讯作者：
Chai, Renjie
中科院分区：
文献类型：
--
作者：
Fang, Qiaojun;Zhang, Yuhua;Chai, Renjie
石墨烯由单层或几层厚的二维sp2键合碳片组成，由于其良好的导电性和生物相容性，近年来引起了人们的极大兴趣。三维石墨烯泡沫（3DG）已被证明是用于体外培养神经干细胞（NSC）的稳健支架，其不仅支持NSC生长，而且使细胞保持在比2D石墨烯膜和普通玻璃更活跃的增殖状态。此外，3DG可以促进NSCs向星形胶质细胞特别是神经元的分化。然而，3DG效应背后的潜在机制仍然知之甚少。新陈代谢是生命的基本特征，为细胞的构建和动力提供物质。代谢活动与干细胞的增殖、分化和自我更新密切相关。本研究主要探讨3DG诱导干细胞代谢重构的机制。本研究建立了代谢重构代谢组学与神经干细胞在不同支架上增殖率的相关性。神经干细胞的增殖变化与多种代谢过程有关。特别是，在3DG上培养触发了增加氨基酸掺入和增强葡萄糖代谢的途径。这些数据表明石墨烯与帕金森病相关通路之间存在潜在关联。我们的工作提供了一个非常有用的起点，为进一步研究的NSC的命运决定3DG。
Graphene consists of two-dimensional sp2-bonded carbon sheets, a single or a few layers thick, which has attracted considerable interest in recent years due to its good conductivity and biocompatibility. Three-dimensional graphene foam (3DG) has been demonstrated to be a robust scaffold for culturing neural stem cells (NSCs) in vitro that not only supports NSCs growth, but also maintains cells in a more active proliferative state than 2D graphene films and ordinary glass. In addition, 3DG can enhance NSCs differentiation into astrocytes and especially neurons. However, the underlying mechanisms behind 3DG's effects are still poorly understood. Metabolism is the fundamental characteristic of life and provides substances for building and powering the cell. Metabolic activity is tightly tied with the proliferation, differentiation, and self-renewal of stem cells. This study focused on the metabolic reconfiguration of stem cells induced by culturing on 3DG. This study established the correlation between metabolic reconfiguration metabolomics with NSCs cell proliferation rate on different scaffold. Several metabolic processes have been uncovered in association with the proliferation change of NSCs. Especially, culturing on 3DG triggered pathways that increased amino acid incorporation and enhanced glucose metabolism. These data suggested a potential association between graphene and pathways involved in Parkinson's disease. Our work provides a very useful starting point for further studies of NSC fate determination on 3DG.