Bridging the neighbor plasma coupling on curved surface array for early hepatocellular carcinoma detection
Bridging the neighbor plasma coupling on curved surface array for early hepatocellular carcinoma detection
复制标题
桥接曲面阵列上的邻近等离子体耦合用于早期肝细胞癌检测
DOI：
10.1016/j.snb.2020.127759
复制
发表时间：
2020-04-15
影响因子：
8.4
通讯作者：
Zhang, Xuefeng
中科院分区：
文献类型：
--
作者：
Cheng, Mingyu;Zhang, Fan;Zhang, Xuefeng
高灵敏度的生物标志物检测是肝细胞癌（HCC）早期诊断的关键。表面粗糙的纳米结构单元被桥接，获得了显著的表面增强拉曼散射（Sers）信号。这改变了等离子体耦合模式和相邻单元之间的热点分布，如时域有限差分模拟所证实的。这些桥接的多节单元阵列用于通过使用Sers的频移和强度变化的组合的免疫分析来定量分析甲胎蛋白（AFP）和AFP-L3的浓度。将这些桥联多节单元阵列用作Sers免疫芯片，AFP和AFP-L3的检测限降低到3 pg/mL。该免疫分析方法具有简便、快速、检出限低、精密度高等优点，对早期肝癌的诊断具有潜在的应用价值。
Detecting biomarkers with high sensitivity is key for the early diagnosis of hepatocellular carcinoma (HCC). Nanostructure units with knobby surface are bridged to obtain significant surface-enhanced Raman scattering (SERS) signals. This changes the plasma coupling mode and the hotspots distribution between neighboring units as confirmed by finite-difference time-domain simulations. These arrays of bridged knobby units are used to quantitatively analyze the concentrations of alpha-Fetoprotein (AFP) and AFP-L3 by immunoanalysis using a combination of frequency shifts and intensity changes of SERS. By using these arrays of bridged knobby units as SERS immunochips, the detection limits of AFP and AFP-L3 decrease to 3 pg/mL. This immunoanalytic method has potential value for the diagnosis of early liver cancer in patients, offering advantages of convenience, time saving, low detection limit, and high precision.