GENOMIC ORGANIZATION OF BORNA-DISEASE VIRUS
GENOMIC ORGANIZATION OF BORNA-DISEASE VIRUS
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DOI：
10.1073/pnas.91.10.4362
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发表时间：
1994-05-10
影响因子：
11.1
通讯作者：
LIPKIN, WI
中科院分区：
文献类型：
--
作者：
BRIESE, T;SCHNEEMANN, A;LIPKIN, WI
博尔纳病病毒是一种嗜神经性负链 RNA 病毒，可感染多种脊椎动物宿主，导致运动和行为障碍。我们利用博尔纳病病毒颗粒的 RNA 克隆并测序了 8910 个核苷酸的病毒基因组。病毒基因组具有互补的 3' 和 5' 末端，并包含五个开放阅读框的反义信息。在顺反子和顺反子外区域均发现与丝状病毒科、副粘病毒科和弹状病毒科的同源性。 Northern 分析表明，该病毒转录单顺反子和多顺反子 RNA，并使用终止/聚腺苷酸化信号，这让人想起在其他负链 RNA 病毒中观察到的信号。博尔纳病病毒很可能代表了单负病毒目中以前未被识别的博尔纳病毒属或博尔纳病毒科。
Borna disease virus is a neurotropic negative-strand RNA virus that infects a wide range of vertebrate hosts, causing disturbances in movement and behavior. We have cloned and sequenced the 8910-nucleotide viral genome by using RNA from Borna disease virus particles. The viral genome has complementary 3' and 5' termini and contains antisense information for five open reading frames. Homology to Filoviridae, Paramyxoviridae, and Rhabdoviridae is found in both cistronic and extracistronic regions. Northern analysis indicates that the virus transcribes mono- and polycistronic RNAs and uses termination/polyadenylylation signals reminiscent of those observed in other negative-strand RNA viruses. Borna disease virus is likely to represent a previously unrecognized genus, bornaviruses, or family, Bornaviridae, within the order Mononegavirales.