Micro-MRI methods to detect renal cysts in mice
Micro-MRI methods to detect renal cysts in mice
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DOI：
10.1111/j.1523-1755.2004.00532.x
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发表时间：
2004-04-01
影响因子：
19.6
通讯作者：
Star, RA
中科院分区：
文献类型：
--
作者：
Kobayashi, H;Kawamoto, S;Star, RA
背景小鼠疾病模型，特别是使用转基因和基因敲除技术，是分析疾病分子基础的有力工具。我们最近报道了一种新的动态micro-MRI方法与树枝状聚合物为基础的造影剂，可以可视化肾脏的结构和功能，在正常的生活小鼠和小鼠急性肾功能衰竭。虽然MRI对比增强可用于检测肾脏的功能障碍，但该技术在评估形态学变化，特别是囊性疾病方面存在局限性，因为对比增强的micro-MRI将囊肿描绘为低强度区域，无法与纤维化灶区分开来。在本研究中，我们评估了采用新的三维MR水成像信号序列[采用稳态采集的三维快速成像（3D-FIESTA）]的micro-MRI是否可以在不使用任何造影剂的情况下显示慢性囊性病变。我们能够在镰状细胞病小鼠模型中明确描述多个直径约0.2 mm的肾皮质囊肿，并在2个半月的时间内观察到环氧化酶-2（考克斯-2）基因敲除小鼠肾囊肿（直径约0.2 mm）的系列变化。一些囊肿随着时间的推移而缩小。3D-FIESTA的Micro-MRI可以在不注射造影剂的情况下描绘活小鼠的患病肾脏中的囊肿形成。
Background. Mouse models of disease, especially using transgenic and knockout technologies, are powerful tools to analyze the molecular basis of disease. We recently reported that a new dynamic micro-MRI method with dendrimer-based contrast agents can visualize renal structure and function in normal living mice and mice with acute renal failure. While MRI contrast enhancement is useful for detecting functional impairment of the kidneys, this technology has limitations in assessing morphologic changes, particularly cystic disease, because contrast-enhanced micro-MRI depicts cysts as low-intensity areas that cannot be distinguished from fibrotic foci.Methods. In the current study, we evaluated if micro-MRI employing a new three-dimensional MR hydrography signal sequence [three-dimensional fast imaging employing steady-state acquisition (3D-FIESTA)] can visualize chronic cystic changes without any contrast agents.Results. We were able to positively depict multiple renal cortical cysts of similar to0.2 mm diameter in a mouse model of sickle cell disease and observe serial changes of renal cysts ( 0.2 mm diameter) in cyclooxygenase-2 (COX-2) knockout mice during a 2 1/2-month period. Some cysts decreased in size over time.Conclusions. Micro-MRI with 3D-FIESTA can depict cyst formation in the diseased kidneys of living mice without injection of contrast agents.