Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-metastatic murine colon 26-L5 carcinoma cell line
Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-metastatic murine colon 26-L5 carcinoma cell line
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DOI：
10.1016/s0968-0896(02)00138-4
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发表时间：
2002-10-01
影响因子：
3.5
通讯作者：
Kadota, S
中科院分区：
文献类型：
--
作者：
Nagaoka, T;Banskota, AH;Kadota, S
制备了咖啡酸苯乙酯（CAPE, 2）及其20个类似物（1,3 -21）。采用MTT法对小鼠结肠26-L5癌、小鼠B16-BL6恶性瘤、小鼠Lewis肺癌、人HT-1080纤维肉瘤、人肺A549腺癌和人宫颈HeLa腺癌细胞株的生长进行了检测。发现CAPE类似物对小鼠高肝转移结肠癌26-L5细胞系具有选择性的抗增殖活性。其中4-苯基咖啡酸丁酯(4)、(Z)-8-苯基-7-辛烯基（10a）和(E)-8-苯基-7-辛烯基（10b）咖啡酸酯的抗增殖活性最强（EC50值为0.02 muM）。此外，CAPE(2)在浓度为1 ~ 10马克杯/毫升时诱导小鼠结肠26-L5癌细胞DNA断裂。(C) 2002 Elsevier Science Ltd.版权所有。
Caffeic acid phenethyl ester (CAPE, 2) and its twenty analogues (1, 3-21) were prepared. These esters were tested by MTT assay on growth of murine colon 26-L5 carcinoma, murine B16-BL6 malonoma, murine Lewis lung carcinoma, human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma, and human cervix HeLa adenocarcinoma cell lines. It was found that CAPE analogues possessed selective antiproliferative activity toward highly liver-metastatic murine colon 26-L5 carcinoma cell line. Among them, 4-phenylbutyl caffeate (4), (Z)-8-phenyl-7-octenyl (10a) and (E)-8-phenyl-7-octenyl (10b) caffeate showed the most potent antiproliferative activity (EC50 value, 0.02 muM). In addition, CAPE (2) induced DNA fragmentation at concentrations of 1 to 10 mug/mL towards murine colon 26-L5 carcinoma cells. (C) 2002 Elsevier Science Ltd. All rights reserved.