Characterization of a formaldehyde degrading fungus Penicillium chrysogenum DY-F2 isolated from deep sea sediment

Characterization of a formaldehyde degrading fungus Penicillium chrysogenum DY-F2 isolated from deep sea sediment
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从深海沉积物中分离出的甲醛降解真菌 Penicillium chrysogenum DY-F2 的表征

DOI:
10.1016/j.ibiod.2013.12.019
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发表时间:
2014-04
影响因子:
4.8
通讯作者:
Zhu-Hua Luo
Zhu-Hua Luo
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Jing-Jing Luo;Jie-Fei Ding;Guang-Wei Li;Tian-Ling Zheng;Zhu-Hua Luo

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采用富集培养技术从东太平洋深海沉积物中分离到一株甲醛降解菌,经显微孢子形态和18S rRNA基因序列分析鉴定为产黄青霉DY-F2。该真菌表现出较高的耐甲醛能力,能在L−1的甲醛存在下生长,最适温度为25℃,在L−1的甲醛存在下生长的最适pH为6.0。该真菌能够降解甲醛作为唯一的碳源和能源,并以甲酸的形成为中间产物。该菌对甲醛的降解符合一级动力学模型。本研究表明,深海沉积物真菌是海洋环境中甲醛污染生物修复的潜在微生物资源。
A formaldehyde-degrading fungus was isolated from deep sea sediment of East Pacific by enrichment culture technique and was identified asPenicillium chrysogenumDY-F2 based on microscopic spore morphology and 18S rRNA gene sequence analysis. The fungus showed high formaldehyde resistance and was able to grow in the presence of formaldehyde up to 3000 mg l−1. The optimal temperature and pH for the growth of fungus in the presence of 1000 mg l−1of formaldehyde was 25 °C and 6.0, respectively. The fungus was able to degrade formaldehyde as the sole source of carbon and energy with the formation of formic acid as the intermediate. Degradation of formaldehyde by the fungus conformed to a first-order kinetic model. This study showed that the deep sea sediment fungi are the potential microbial resources for bioremediation of formaldehyde pollution in marine environment.
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影响因子: 5.8
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