Identification of Circular RNAs and Their Targets in Leaves of Triticum aestivum L. under Dehydration Stress.
Identification of Circular RNAs and Their Targets in Leaves of Triticum aestivum L. under Dehydration Stress.
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DOI：
10.3389/fpls.2016.02024
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发表时间：
2016
影响因子：
5.6
通讯作者：
Suo B
中科院分区：
文献类型：
--
作者：
Wang Y;Yang M;Wei S;Qin F;Zhao H;Suo B
环状RNAs(CircRNAs)是通过高通量深度测序新发现的一类非编码RNA，在人类、动物和植物的miRNA功能和转录调控中发挥着重要作用。到目前为止，在小麦幼苗中还没有关于CircRNAs的鉴定及其在脱水胁迫反应中的作用的报道。在本研究中，分别从脱水胁迫和水分充足条件下的小麦幼苗叶片中提取总RNA。然后，对CircRNAs富集库进行深度测序，并利用生物信息学工具对CircRNAs进行鉴定。在小麦幼苗叶片中分离到大约88个候选CircRNAs，其中62个在脱水胁迫下与水分充足的对照相比差异表达。在对脱水反应敏感的CircRNAs中，有6个在小麦中被发现作为26个相应的miRNAs海绵。实时荧光定量PCR进一步验证了包括6个miRNAs海绵在内的16个CircRNAs和其他10个随机选择的CircRNAs是环状的，其中14个与转录组测序结果显示出一致的调控模式。经过基因本体论(GO)和京都基因和基因组百科全书(KEGG)对目标mRNAs功能的分析，这些CircRNAs被预测参与了脱水反应过程，如光合作用、卟啉和叶绿素代谢、氧化磷酸化、氨基酸生物合成和代谢，以及植物激素信号转导，包括生长素、油菜素类固醇和水杨酸。在此，我们揭示了CircRNAs的调控与小麦叶片中与脱水抗性相关的功能基因表达之间的可能联系。
Circular RNAs (circRNAs) are a type of newly identified non-coding RNAs through high-throughput deep sequencing, which play important roles in miRNA function and transcriptional controlling in human, animals, and plants. To date, there is no report in wheat seedlings regarding the circRNAs identification and roles in the dehydration stress response. In present study, the total RNA was extracted from leaves of wheat seedlings under dehydration-stressed and well-watered conditions, respectively. Then, the circRNAs enriched library based deep sequencing was performed and the circRNAs were identified using bioinformatics tools. Around 88 circRNAs candidates were isolated in wheat seedlings leaves while 62 were differentially expressed in dehydration-stressed seedlings compared to well-watered control. Among the dehydration responsive circRNAs, six were found to act as 26 corresponding miRNAs sponges in wheat. Sixteen circRNAs including the 6 miRNAs sponges and other 10 randomly selected ones were further validated to be circular by real-time PCR assay, and 14 displayed consistent regulation patterns with the transcriptome sequencing results. After Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the targeted mRNAs functions, the circRNAs were predicted to be involved in dehydration responsive process, such as photosynthesis, porphyrin, and chlorophyll metabolism, oxidative phosphorylation, amino acid biosynthesis, and metabolism, as well as plant hormone signal transduction, involving auxin, brassinosteroid, and salicylic acid. Herein, we revealed a possible connection between the regulations of circRNAs with the expressions of functional genes in wheat leaves associated with dehydration resistance.
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影响因子：
14.9
作者：
Danan M;Schwartz S;Edelheit S;Sorek R
通讯作者：
Sorek R
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
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作者：
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水稻环状RNA的全转录组研究
DOI：
10.1261/rna.052282.115
发表时间：
2015-12
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Lu T;Cui L;Zhou Y;Zhu C;Fan D;Gong H;Zhao Q;Zhou C;Zhao Y;Lu D;Luo J;Wang Y;Tian Q;Feng Q;Huang T;Han B
通讯作者：
Han B
影响因子：
3.7
作者：
Salzman J;Gawad C;Wang PL;Lacayo N;Brown PO
通讯作者：
Brown PO