Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family
Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family
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DOI：
10.1016/j.tox.2006.10.020
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发表时间：
2007-01-18
期刊：
影响因子：
4.5
通讯作者：
Huang, Jian
中科院分区：
文献类型：
--
作者：
Luo, Cong;Zhu, Yongliang;Huang, Jian
苦参碱是苦参干燥根的主要活性成分之一，在体外可诱导多种肿瘤细胞凋亡。然而，苦参碱诱导细胞凋亡的分子机制仍不清楚。在此，我们研究了苦参碱对人胃癌MKN45细胞的诱导凋亡作用。结果表明，苦参碱具有抑制细胞增殖和诱导细胞凋亡的作用，且呈剂量依赖性。进一步的免疫印迹显示，在苦参碱处理的细胞中，caspase-3、-7被激活，促凋亡分子Bok、Bak、Bax、Puma和Bim也上调。我们的结果提示，苦参碱通过增加Bcl2家族促凋亡分子诱导胃癌MKN45细胞凋亡。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. (c) 2006 Elsevier Ireland Ltd. All rights reserved.