Graphically encoded suspension array for multiplex immunoassay and quantification of autoimmune biomarkers in patient sera
Graphically encoded suspension array for multiplex immunoassay and quantification of autoimmune biomarkers in patient sera
复制标题
用于多重免疫测定和患者血清中自身免疫生物标志物定量的图形编码悬浮阵列
DOI：
10.1016/j.bios.2019.02.030
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发表时间：
2019-05-01
影响因子：
12.6
通讯作者：
Li，Jiong
中科院分区：
文献类型：
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作者：
Zheng，Kexiao;Chen，Chao;Li，Jiong
在精准医学中，临床决策和药物评估往往是在对多种蛋白质生物标志物进行平行分析的基础上做出的。目前，光谱编码珠流悬浮阵列和其他平台部分满足了对高通量多重免疫测定的日益增长的需求，但在编码能力、解码准确性、易于制造/操作和成本效益方面仍有进步的空间，图形悬浮阵列可以为此做出实质性贡献。在这里，我们描述了一个悬浮阵列系统由图形编码的二氧化硅颗粒，一个自动化的微孔板成像仪和一个内部的数据处理程序。微加工的，高度均匀的平面粒子提供了一个128重的代码空间，进一步扩展。衍生的多重免疫分析达到了亚皮克/毫升的灵敏度水平（最低LoD = 80 fg/ml），具有宽的动态范围，以及高的精密度和准确度。通过平行测量1型糖尿病患者的三种血清生物标志物，证明了临床诊断的潜力。重要的是，使用标准微孔板作为测定容器将其能力扩展到高通量应用，例如疾病筛查或药物发现。
In precision medicine, clinical decisions and pharmaceutical evaluations tends to be made upon parallel analysis of multiple protein biomarkers. Currently, the growing needs of high-throughput multiplex immunoassay is partially satisfied by spectrally encoded bead flow suspension arrays and other platforms, yet there is still room for progress in terms of encoding capacity, decoding accuracy, ease-of-manufacture/operation, and cost-effectiveness, for which graphical suspension arrays could make substantial contributions. Here we described a suspension array system made up of graphically encoded silica particles, an automated microplate imager and an in-house data processing program. The micro-fabricated, highly uniform planar particles provide a code space of 128-plex with further extendibility. The derived multiplex immunoassay reaches sub-picogram per milliliter sensitivity level (lowest LoD = 80 fg/ml) with wide dynamic range, as well as high precision and accuracy. The potential of clinical diagnostics was demonstrated by parallel measurement of three serum biomarkers for type 1 diabetes patients. Importantly, use of standard microplates as assay vessel extends its power to high-throughput applications, such as disease screening or drug discovery.