Condom use and vaginal Y-chromosome detection: The specificity of a potential biomarker
Condom use and vaginal Y-chromosome detection: The specificity of a potential biomarker
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DOI：
10.1097/01.olq.0000258318.99606.d9
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发表时间：
2007-08-01
影响因子：
3.1
通讯作者：
Zenilman, Jonathan
中科院分区：
文献类型：
--
作者：
Ghanem, Khalil G.;Melendez, Johan H.;Zenilman, Jonathan
目的：阴道y染色体序列（YCS）检测可能是验证女性性行为报告的有用生物标志物。我们描述了避孕套使用对阴道YCS检测的影响。方法：选择56名妇女禁欲14天。在第15天，参与者被要求使用避孕套与他们的男性伴侣发生性关系。于第14、16和17天自行收集阴道拭子。使用Roche LightCycler检测YCS，并使用阳性对照。结果：56名女性中有44人完成了研究。5名妇女（11.4%）有可检出的YCS。使用安全套的YCS检测的总特异性为92% （95% CI: 80%-98%）。尽管报告在48小时内接受口交和手指插入的妇女的YCS检出率较高[RR分别为2.3 （95% CI: 1.1-4.6）和3.6 (95% CI: 1.6-8.5)]，但YCS的平均浓度远低于无保护阴道性交的妇女
Objective: Detection of vaginal Y-chromosome sequences (YCS) may be a useful biomarker to validate sexual behavior reporting in women. We describe the effects of condom use on the detection of vaginal YCS.Methods: Fifty-six women were asked to abstain from sexual intercourse for 14 days. On day 15, participants were asked to engage in sexual intercourse with their male partners using condoms. Self-collected vaginal swabs were obtained on days 14, 16, and 17. YCS were detected using the Roche LightCycler with the use of positive controls.Results: Fourty-four of 56 women completed the study. Five women (11.4%) had detectable YCS. The overall specificity of the YCS assay with condom use was 92% (95% CI: 80%-98%). Although women who reported receptive oral sex and digital penetration within 48 hours of swab collection had a higher detection rate of YCS [RR 2.3 (95% CI: 1.1-4.6) and 3.6 (95% CI: 1.6-8.5), respectively], the mean concentration of YCS was much less than that associated with unprotected vaginal intercourse (P