Discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus Gyrovirus
Discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus Gyrovirus
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DOI：
10.1007/s00705-011-0971-6
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Roehe, Paulo M.
中科院分区：
文献类型：
--
作者：
Rijsewijk, Franciscus A. M.;dos Santos, Helton F.;Roehe, Paulo M.
从病鸡血清中克隆了一条2.4kb的phi29聚合酶扩增产物，并对其进行了序列分析。2383个核苷酸序列与鸡贫血病毒(CAV)的一个代表性基因组有约40%的同源性，CAV是圆环病毒科GyroVirus的唯一成员。新的基因组具有与CAV相似的结构：假定有一个约400nT的5‘非转录区域，其后是编码VP1、VP2和VP3同源物的三个部分重叠的开放阅读框。与CAV的氨基酸同源性分别为38.8%、40.3%和32.2%。根据这些有限的相似性，推测新发现的病毒是GyroVirus属的一个新种。对这一新种，建议命名为禽回旋病毒2(AGV2)。
A 2.4-kb phi29 polymerase amplification product from serum of a diseased chicken was cloned and sequenced. The 2383-nucleotide sequence showed about 40% identity to a representative genome of chicken anemia virus (CAV), the only member of the genus Gyrovirus, family Circoviridae. The new genome had an organization similar to that of CAV: a putative 5' untranscribed region of about 400 nt followed by three partially overlapping open reading frames encoding VP1, VP2 and VP3 homologs. The amino acid identities between these homologs and those of CAV were 38.8%, 40.3%, and 32.2%, respectively. Based on these limited similarities, it is proposed that the newly identified virus is a member of a new species in the genus Gyrovirus. For this new species, the name Avian gyrovirus 2 (AGV2) is proposed.