Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration
Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration
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DOI：
10.1002/adhm.202202611
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发表时间：
2023-01-26
影响因子：
10
通讯作者：
Huang，Cui
中科院分区：
文献类型：
--
作者：
Wu，Xiaoyi;Peng，Wenan;Huang，Cui
牙本质是一种天然的细胞外基质，但由于缺乏适当的治疗，其在骨移植和组织工程应用中的可用性被低估。本研究首次将原纤维外脱矿的概念引入牙本质衍生骨再生生物材料的构建中。具有大分子量的钙螯合剂用于选择性地去除纤维外磷灰石矿物质，而不干扰牙本质胶原内的纤维内矿物质，导致纤维外脱矿牙本质基质（EDM）的形成。具有独特的纳米形貌和骨样机械性能的EDM被发现在体外显著促进细胞粘附、迁移和成骨分化，同时增强大鼠颅骨缺损的体内骨愈合。进一步证实了EDM突出的成骨性能与激活粘着斑-细胞骨架-细胞核力学传导轴有关。总的来说，这项研究表明，牙本质的纤维外脱矿具有很大的潜力，可以产生用于骨再生的分级胶原基支架，这种简单的自上而下的制造方法为天然生物活性材料的生物医学应用带来了新的思路。
Dentin is a natural extracellular matrix, but its availability in bone grafting and tissue engineering applications is underestimated due to a lack of proper treatment. In this study, the concept of extrafibrillar demineralization is introduced into the construction of dentin‐derived biomaterials for bone regeneration for the first time. Calcium chelating agents with large molecular weights are used to selectively remove the extrafibrillar apatite minerals without disturbing the intrafibrillar minerals within dentin collagen, resulting in the formation of an extrafibrillarly demineralized dentin matrix (EDM). EDM with distinctive nanotopography and bone‐like mechanical properties is found to significantly promote cell adhesion, migration, and osteogenic differentiation in vitro while enhancing in vivo bone healing of rat calvarial defects. The outstanding osteogenic performance of EDM is further confirmed to be related to the activation of the focal adhesion–cytoskeleton–nucleus mechanotransduction axis. Overall, this study shows that extrafibrillar demineralization of dentin has great potential to produce hierarchical collagen‐based scaffolds for bone regeneration, and this facile top‐down fabrication method brings about new ideas for the biomedical application of naturally derived bioactive materials.