Roles of innate and adaptive immunity in respiratory mycoplasmosis
Roles of innate and adaptive immunity in respiratory mycoplasmosis
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DOI：
10.1128/iai.66.8.3485-3491.1998
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发表时间：
1998-08-01
影响因子：
3.1
通讯作者：
Simecka, JW
中科院分区：
文献类型：
--
作者：
Cartner, SC;Lindsey, JR;Simecka, JW
目前的证据表明，呼吸道支原体病的宿主防御依赖于先天免疫和体液免疫。为了进一步阐明先天性和适应性免疫在抗支原体防御中的作用，我们鼻内感染C3 H/HeSnJ-scid/scid。（C3H-SCID），C3H/HeSnJ（C3H），C57BL/6J-scid/scid（C57-SCID）和C57 BL/6 N（C57 BL）小鼠感染肺支原体，并在感染后14和21天进行肺和脾的定量培养，肺病变的定量，以及所有其他主要器官的组织病理学评估。我们发现肺中支原体的数量与遗传背景（C3 H易感，C57 BL抗性）而不是适应性免疫的功能状态相关，表明先天免疫是肺抗支原体防御的主要贡献者。肺外支原体播散伴脾脏定植和多器官组织学病变在所有小鼠中均常见。严重联合免疫缺陷（SCID）小鼠缺乏适应性免疫应答，导致脾脏支原体定植增加，肺外部位（特别是脾脏、心脏和关节）病变增加，肺病变严重程度降低。抗M.肺结核血清对感染的C3 H-SCID小鼠预防了肺外感染和疾病，而通过将幼稚脾细胞转移到感染的C3 H-SCID小鼠中恢复了肺损伤的严重程度。总的来说，我们的研究结果强烈支持的结论，先天免疫提供抗支原体防御的肺和体液免疫的主要作用，在防御系统传播的支原体感染，但细胞免疫反应可能是重要的支原体肺病的恶化。
Current evidence suggests that host defense in respiratory mycoplasmosis is dependent on both innate and humoral immunity. To further delineate the roles of innate and adaptive immunity in antimycoplasmal defenses, we intranasally infected C3H/HeSnJ-scid/scid (C3H-SCID), C3H/HeSnJ (C3H), C57BL/6J-scid/scid (C57-SCID), and C57BL/6N (C57BL) mice with Mycoplasma pulmonis and at 14 and 21 days postinfection performed quantitative cultures of lungs and spleens, quantification of lung lesions, and histopathologic assessments of all other major organs. We found that numbers of mycoplasmas in lungs were associated with genetic background (C3H susceptible, C57BL resistant) rather than functional state of adaptive immunity, indicating that innate immunity is the main contributor to antimycoplasmal defense of the lungs. Extrapulmonary dissemination of mycoplasmas with colonization of spleens and histologic lesions in multiple organs was a common occurrence in all mice. The absence of adaptive immune responses in severe combined immunodeficient (SCID) mice resulted in increased mycoplasmal colonization of spleens and lesions in extrapulmonary sites, particularly spleens, hearts, and joints, and also reduced lung lesion severity. The transfer of anti-M. pulmonis serum to infected C3H-SCID mice prevented extrapulmonary infection and disease, while the severity of lung lesions was restored by transfer of naive spleen cells to infected C3H-SCID mice. Collectively, our results strongly support the conclusions that innate immunity provides antimycoplasmal defense of the lungs and humoral immunity has the major role in defense against systemic dissemination of mycoplasmal infection, but cellular immune responses may be important in exacerbation of mycoplasmal lung disease.