A REPETITIVE DNA-SEQUENCE THAT CONFERS CELL-CYCLE START (CDC28)-DEPENDENT TRANSCRIPTION OF THE HO GENE IN YEAST
A REPETITIVE DNA-SEQUENCE THAT CONFERS CELL-CYCLE START (CDC28)-DEPENDENT TRANSCRIPTION OF THE HO GENE IN YEAST
复制标题
DOI：
10.1016/s0092-8674(85)80118-5
复制
发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
NASMYTH, K
中科院分区：
文献类型：
--
作者：
NASMYTH, K
酵母中交配型的相互转化是由HO基因编码的核酸内切酶启动的。HO在细胞周期的G1期仅瞬时转录，因为细胞经历START。HO 5“-侧翼DNA的缺失分析表明，在-150到-900的区间内，必须有多个拷贝的序列赋予START特异性转录。该间隔的分析揭示了10个与共有Pur N N Pyr C A C G A4紧密匹配的序列的出现。为了测试这些序列是否是推定的细胞周期控制元件，在组成性缺失的断点处插入合成衍生的共有序列拷贝，并显示恢复START依赖性细胞周期控制。
The interconversion of mating types in yeast is initiated by an endonuclease encoded by the HO gene. HO is transcribed only transient during the G1 phase of the cell cycle, as cells undergo START. A deletion analysis of HO 5''-flanking DNA suggests that there must be multiple copies of the sequence that confers START-specific transcription in the interval from -150 to -900. Analysis of this interval revealed 10 occurrences of sequences that closely match the consensus Pur N N Pyr C A C G A4. To test whether these sequences are the putative cell-cycle control elements, synthetically derived copies of the consensus were inserted at the break points of constitutive deletions and shown to restore START-dependent cell-cycle control.