Grape Seed Extract Induces Cell Cycle Arrest and Apoptosis in Human Colon Carcinoma Cells
Grape Seed Extract Induces Cell Cycle Arrest and Apoptosis in Human Colon Carcinoma Cells
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DOI：
10.1080/01635580802381295
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Agarwal, Chapla
中科院分区：
文献类型：
--
作者：
Kaur, Manjinder;Mandair, Reinuka;Agarwal, Chapla
控制结直肠癌（CRC）的一种方法是通过饮食制剂或作为补充剂食用的预防性干预。但是，由于这些产品大多数经常被患者作为补充和替代医学实践使用，因此需要开发疗效、机制和标准化制剂等科学基础。葡萄籽提取物（GSE）是一种因其多种健康益处而被人类广泛食用的补充剂。我们最近报道GSE抑制大肠癌细胞HT 29在培养和裸鼠移植瘤中的生长。由于GSE可通过不同的供应商购获得，因此我们评估了来自2个不同制造商的GSE是否在一组人CRC细胞系中产生相当的生物学效应。我们的研究结果表明，无论来源，GSE强烈抑制LoVo，HT 29，和SW 480细胞的生长，与G1停滞在LoVo和HT 29细胞，但S和/或G2/M停滞在SW 480细胞周期进程。GSE还在所有3种细胞系中诱导Cip/p21水平。此外，在所有3个细胞系中观察到GSE诱导凋亡。综上所述，我们的研究结果表明，GSE可能是一种有效的CAM剂对CRC可能是由于其强大的生长抑制和诱导凋亡的作用。
One approach to control colorectal cancer (CRC) is its preventive intervention by dietary agents or those consumed as supplements. However, because most of these products are often consumed by patients as an complementary and alternative medicine practice, a scientific base such as efficacy, mechanism, and standardized preparation needs to be developed. Grape seed extract (GSE) is one such supplement widely consumed by humans for its several health benefits. We reported recently that GSE inhibits CRC cell HT29 growth in culture and nude mice xenograft. Because GSE is available commercially through different vendors, here we assessed whether GSE from 2 different manufacturers produces comparable biological effects in a panel of human CRC cell lines. Our results show that irrespective of source, GSE strongly inhibits LoVo, HT29, and SW480 cell growth, with a G1 arrest in LoVo and HT29 cells but an S and/or G2/M arrest in SW480 cell cycle progression. GSE also induced Cip/p21 levels in all 3 cell lines. Furthermore, an induction of apoptosis was observed in all 3 cell lines by GSE. Taken together, our findings suggest that GSE could be an effective CAM agent against CRC possibly due to its strong growth inhibitory and apoptosis-inducing effects.