Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution
Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution
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DOI：
10.1242/dev.01289
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发表时间：
2004-09-01
期刊：
影响因子：
4.6
通讯作者：
Schmidt-Ott, U
中科院分区：
文献类型：
--
作者：
Bullock, SL;Stauber, M;Schmidt-Ott, U
果蝇合胞胚胚节段模式的建立依赖于成对规则的转录调控因子。配对规则基因的mRNA转录本通过基于动力蛋白的选择性运输系统定位到胚皮的顶端细胞质，并在其3'-非翻译区发出信号。然而，这一过程的功能和进化意义尚不清楚。我们分析了来自多个双翅目物种的mrna的亚细胞定位，包括原位和注射到果蝇胚胎中。我们发现，尽管无翅转录本的定位在双翅目中是保守的，但甚至跳过和有毛的成对规则转录本的定位在进化上是不稳定的，并且与分类群特异性的细胞核定位变化有关。我们在果蝇中发现，局部配对规则转录本在靠近细胞核的地方靶向它们的蛋白质，并通过增加基因活性来提高分割过程的可靠性。我们的数据表明，配对规则转录本中的mRNA定位信号影响核蛋白摄取，从而调节基因活性以适应各种双翅类囊胚细胞结构。
Establishment of segmental pattern in the Drosophila syncytial blastoderm embryo depends on pair-rule transcriptional regulators. mRNA transcripts of pair-rule genes localise to the apical cytoplasm of the blastoderm via a selective dynein-based transport system and signals within their 3'-untranslated regions. However, the functional and evolutionary significance of this process remains unknown. We have analysed subcellular localisation of mRNAs from multiple dipteran species both in situ and by injection into Drosophila embryos. We find that although localisation of wingless transcripts is conserved in Diptera, localisation of even-skipped and hairy pair-rule transcripts is evolutionarily labile and correlates with taxon-specific changes in positioning of nuclei. We show in Drosophila that localised pair-rule transcripts target their proteins in close proximity to the nuclei and increase the reliability of the segmentation process by augmenting gene activity. Our data suggest that mRNA localisation signals in pair-rule transcripts affect nuclear protein uptake and thereby adjust gene activity to a variety of dipteran blastoderm cytoarchitectures.