Correlation between Systemic Oxidative Stress and Intraocular Pressure Level.
Correlation between Systemic Oxidative Stress and Intraocular Pressure Level.
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DOI：
10.1371/journal.pone.0133582
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ohira A
中科院分区：
文献类型：
--
作者：
Tanito M;Kaidzu S;Takai Y;Ohira A
在青光眼的发病机制中，局部和全身氧化应激参与了眼内压升高和视神经损伤的假设。我们以前报道过，开角型青光眼患者的全身抗氧化能力水平低于无青光眼的对照组。在这里，我们通过分析青光眼患者的血液生化来评估IOP与全身促氧化剂和抗氧化剂水平的相关性。采集日本原发性开角型青光眼（n = 206）、脱落综合征（n = 199）和对照组（n = 126）患者的外周血样本。采用自由基分析仪分别通过diacron活性氧代谢物（dROM）、生物抗氧化电位（BAP）和巯基（SH）试验测定血清脂质过氧化物水平、铁还原活性和硫醇抗氧化活性。为了测试氧化应激对IOP水平的可能影响，根据IOP的四分位数值将患者分为四组（Q1、Q2、Q3和Q4，其中Q1的IOP最低）。在这种分类中，将已知的左右眼最高IOP值作为每个受试者的IOP值。对于IOP组之间的比较，使用单向方差分析和事后非配对t检验计算差异。为了调整人口统计学特征分布的差异，采用多元logistic回归分析比较IOP组间的dROM、BAP和SH检验值；以Q1组为参照计算各变量的比值比（odds ratio， OR）。四组间dROM和SH水平差异无统计学意义（p = 0.6704和p = 0.6376）。四组间BAP水平差异有统计学意义（p = 0.0115）；Q4组（1932 μmol/L）显著低于Q1组（2023 μmol/L, p = 0.0042）和Q2组（2003 μmol/L, p = 0.0302）， Q3组（1948 μmol/L）显著低于Q1组（p = 0.0174）。在调整各种人口统计学特征的差异后，BAP值越低，IOP越高组越显著（Q3组，p = 0.0261, OR = 0.06/range； Q4组，p = 0.0018, OR = 0.04/range）。在任何比较中，dROM和SH值都没有达到显著性。通过铁还原活性测量的较低的全身抗氧化能力通过其在IOP升高中的作用参与了开角型青光眼的发病机制。
The involvement of local and systemic oxidative stress in intraocular pressure (IOP) elevation and optic nerve damage has been hypothesized in the pathogenesis of glaucoma. We reported previously that the level of systemic antioxidative capacity is lower in patients with open-angle glaucoma than controls without glaucoma. Here, we assessed the correlation between IOP and systemic levels of prooxidants and antioxidants by analyzing the blood biochemistry in patients with glaucoma. Peripheral blood samples were collected from Japanese patients with primary open-angle glaucoma (n = 206), exfoliation syndrome (n = 199), and controls (n = 126). Serum levels of lipid peroxides, ferric-reducing activity, and thiol antioxidant activity were measured by diacron reactive oxygen metabolite (dROM), biological antioxidant potential (BAP), and sulfhydryl (SH) tests, respectively, using a free radical analyzer. To test the possible effect of oxidative stress on IOP levels, the patients were classified into one of four groups (Q1, Q2, Q3, and Q4, with Q1 having the lowest IOP) based on the quartile value of IOP. For this classification, the known highest IOP value in both the right and left eyes was regarded as each subject’s IOP. For comparisons among the IOP groups, the differences were calculated using one-way analysis of variance followed by post-hoc unpaired t-tests. To adjust for differences in demographic characteristic distributions, the dROM, BAP, and SH test values were compared among the IOP groups using multiple logistic regression analysis; the odds ratio (OR) of each variable was calculated with the Q1 group as the reference. The dROM and the SH levels did not differ significantly (p = 0.6704 and p = 0.6376, respectively) among the four IOP groups. The BAP levels differed significantly (p = 0.0115) among the four IOP groups; the value was significantly lower in the Q4 group (1,932 μmol/L) compared with the Q1 (2,023 μmol/L, p = 0.0042) and Q2 (2,003 μmol/L, p = 0.0302) groups and significantly lower in the Q3 group (1,948 μmol/L) than the Q1 (p = 0.0174) group. After adjustment for differences in various demographic characteristics, lower BAP values were significantly associated with the classification into higher IOP groups (Q3 group, p = 0.0261 and OR = 0.06/range; Q4 group, p = 0.0018 and OR = 0.04/range). The dROM and SH values did not reach significance in any comparisons. Lower systemic antioxidant capacity measured by ferric-reducing activity is involved in the pathogenesis of open-angle glaucoma via its roles in IOP elevation.