Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018
Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018
复制标题
2013-2018年上海瑞金医院血流感染金黄色葡萄球菌耐药情况及分子流行病学分析
DOI：
10.1038/s41598-020-63248-5
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发表时间：
2020-04-07
影响因子：
4.6
通讯作者：
Han, Lizhong
中科院分区：
文献类型：
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作者：
Gu, Feifei;He, Weiping;Han, Lizhong
金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌（MRSA）是与显著的发病率和死亡率相关的重要问题，并且众所周知是全球引起血流感染（BSI）的主要病原体。了解沙门氏菌的耐药性及分子生物学特征。金黄色葡萄球菌120株。金黄色葡萄球菌分离株（每年20株）从患者的S。随机选择2013年至2018年的金黄色葡萄球菌BSI并入组本研究。53株（44.2%）MRSA分离株被确定，没有发现对万古霉素，达托霉素，增效剂，利奈唑胺和头孢洛林耐药的菌株。与MSSA分离株相比，MRSA分离株中tst、sec、seg和sei毒素基因的频率更高（均P <0.0001）。共鉴定出29种序列类型（ST），ST 5（23.3%）是最常见的ST，其次是ST 398（11.7%）和ST 764（10.0%）。SCCmec II型（73.6%）是MRSA分离株中最常见的SCCmec型。优势克隆复合体（CC）是CC 5（ST 5、ST 764、ST 965和ST 3066; 36.7%）和家畜相关克隆CC 398（ST 398，11.7%）。在53株MRSA中，以CC 5型为主（37/53，69.8%），CC 5型MRSA中有34株为CC 5-Ⅱ型，占91.9%（34/37）。此外，所有29株tst阳性MRSA分离株均为CC 5-MRSA。本研究提供了S. 2013 - 2018年在上海瑞金医院感染的金黄色葡萄球菌引起的BSIs，并可能为进一步研究涉及人类适应性S.金黄色葡萄球菌克隆及其扩散。
Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) is an important issue associated with significant morbidity and mortality and well known as a predominant pathogen causing bloodstream infection (BSIs) globally. To estimate the antibiotic resistance and molecular characteristics of S. aureus causing BSIs in Shanghai, 120 S. aureus isolates (20 isolates each year) from the patients with S. aureus BSIs from 2013 to 2018 were randomly selected and enrolled in this study. Fifty-three (44.2%) MRSA isolates were determined, and no isolate was found resistant to vancomycin, daptomycin, synercid, linezolid and ceftaroline. The toxin genes tst, sec, seg and sei were found more frequently among MRSA isolates compared with MSSA isolates (all P 0.0001). Twenty-nine sequence types (STs) were identified, and ST5 (23.3%) was the most common ST, followed by ST398 (11.7%) and ST764 (10.0%). SCCmec II (73.6%) was the most frequent SCCmec type among MRSA isolates. The dominant clonal complexes (CCs) were CC5 (ST5, ST764, ST965 and ST3066; 36.7%) and the livestock-associated clone CC398 (ST398, 11.7%). MRSA-CC5 was the predominant CC among MRSA isolates (37/53, 69.8%), and CC5-II MRSA was found in 34 isolates accounting for 91.9% (34/37) among CC5 MRSA isolates. In addition, all 29 tst-positive MRSA isolates were CC5-MRSA as well. Our study provided the properties and genotypes of S. aureus causing BSIs at Ruijin Hospital in Shanghai from 2013 to 2018, and might suggest of value clues for the further study insights into pathogenic mechanisms intrinsically referring to the development of human-adapted S. aureus clones and their diffusions.