EPIDERMAL GROWTH-FACTOR INDUCES ACROSOMAL EXOCYTOSIS IN BOVINE SPERM
EPIDERMAL GROWTH-FACTOR INDUCES ACROSOMAL EXOCYTOSIS IN BOVINE SPERM
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DOI：
10.1016/0014-5793(94)80422-2
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发表时间：
1994-02-21
期刊：
影响因子：
3.5
通讯作者：
BREITBART, H
中科院分区：
文献类型：
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作者：
LAX, Y;RUBINSTEIN, S;BREITBART, H
在受精时，哺乳动物精子经历一个钙离子依赖的胞吐事件，这被称为顶体反应(AR)。EGF受体(EGFR)定位于公牛精子头部，表皮生长因子(EGF)以其典型的剂量依赖方式诱导AR的发生。我们先前的研究表明，蛋白激酶C(PKC)参与了导致公牛精子AR的级联反应。在这里，我们证明了PKC参与了EGF对AR的作用机制，这些发现结合我们的结果显示酪氨酸磷酸化抑制剂对AR的抑制作用，表明公牛射出的精子含有一个典型的170 kDa的EGFR，它在导致AR的机制中是活跃的。
At the time of fertilization mammalian spermatozoa undergo a Ca2+-dependent exocytotic event, which is known as the acrosome reaction (AR). We describe here that EGF-receptor (EGFR) is localized in the head of bull spermatozoa and that epidermal growth factor (EGF) can induce the occurrence of the AR in its typical dose-dependent manner. Previously we showed that protein kinase C (PKC) is involved in the cascade leading to AR in bull spermatozoa. Here, we show that PKC is involved in the mechanism in which EGF exerts its effect on AR, These findings together with our results which show inhibition of AR by tyrosine-phosphorylation inhibitors, indicate that ejaculated bull sperm contain a typical 170-kDa EGFR which is active in the mechanism leading to AR.