The characteristics of ubiquitous and unique Leptospira strains from the collection of Russian centre for leptospirosis.
The characteristics of ubiquitous and unique Leptospira strains from the collection of Russian centre for leptospirosis.
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DOI：
10.1155/2014/649034
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发表时间：
2014
影响因子：
--
通讯作者：
Gintsburg AL
中科院分区：
文献类型：
--
作者：
Voronina OL;Kunda MS;Aksenova EI;Ryzhova NN;Semenov AN;Petrov EM;Didenko LV;Lunin VG;Ananyina YV;Gintsburg AL
背景和目标。钩端螺旋体是钩端螺旋体病的病原体，已从俄罗斯的环境、患者和多种动物中分离出来。然而，自然和人为疫源地钩端螺旋体的遗传多样性尚未明确定义。方法。最近的 MLST 方案用于分析七种致病菌。 454焦磷酸测序技术是全基因组测序（WGS）的基础。结果。俄罗斯传播最广、最流行的钩端螺旋体是问号钩端螺旋体、克氏钩端螺旋体和博格彼得森钩端螺旋体。俄罗斯毒株常见的五种 ST：37、17、199、110 和 146，经鉴定在不同地理区域具有长期且普遍的分布。环境钩端螺旋体菌株 Bairam-Ali 揭示了意想不到的特性。该菌株基因组全基因组测序表明，它结合了致病菌株和非致病菌株的特征，可能是天然抗性基因的库。对不同钩端螺旋体菌株的 rrs 和 rpoB 基因以及 MLST 位点以及 Bairam-Ali 菌株的表型和血清学特性进行比较分析的结果表明，它代表了不同的钩端螺旋体菌株。结论。因此，自然和人为疫源地支持了普遍存在的钩端螺旋体物种和对细菌适应各种条件很重要的基因库。
Background and Aim. Leptospira, the causal agent of leptospirosis, has been isolated from the environment, patients, and wide spectrum of animals in Russia. However, the genetic diversity of Leptospira in natural and anthropurgic foci was not clearly defined. Methods. The recent MLST scheme was used for the analysis of seven pathogenic species. 454 pyrosequencing technology was the base of the whole genome sequencing (WGS). Results. The most wide spread and prevalent Leptospira species in Russia were L. interrogans, L. kirschneri, and L. borgpetersenii. Five STs, common for Russian strains: 37, 17, 199, 110, and 146, were identified as having a longtime and ubiquitous distribution in various geographic areas. Unexpected properties were revealed for the environmental Leptospira strain Bairam-Ali. WGS of this strain genome suggested that it combined the features of the pathogenic and nonpathogenic strains and may be a reservoir of the natural resistance genes. Results of the comparative analysis of rrs and rpoB genes and MLST loci for different Leptospira species strains and phenotypic and serological properties of the strain Bairam-Ali suggested that it represented separate Leptospira species. Conclusions. Thus, the natural and anthropurgic foci supported ubiquitous Leptospira species and the pool of genes important for bacterial adaptivity to various conditions.