Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA
Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA
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Musashi-1 游离形式和与 5 聚体 RNA 复合体的第二个 RNA 结合结构域的主链和侧链分配
DOI：
10.1007/s12104-017-9760-9
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发表时间：
2017
影响因子：
0.9
通讯作者：
Katahira Masato
中科院分区：
文献类型：
--
作者：
Iwaoka Ryo;Nagata Takashi;Tsuda Kengo;Imai Takao;Okano Hideyuki;Kobayashi Naohiro;Katahira Masato
Musashi1（Msi1）是一种RNA结合蛋白，参与细胞命运的决定。在这里，我们报告的1H，15 N，和13 C共振分配的Msi 1第二RNA结合结构域的自由形式和与RNA的复合物。分配可用于NMR结构和动力学分析的Msi1：RNA复合物，而且，化学位移扰动分析，以评估潜在的小分子抑制剂对Msi1：RNA相互作用的结合。
Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the1H,15N, and13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.