In vitro galactation of human serum albumin: analysis of the protein's galactation sites by mass spectrometry.
In vitro galactation of human serum albumin: analysis of the protein's galactation sites by mass spectrometry.
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DOI：
10.1016/j.ab.2010.11.034
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发表时间：
2011-03-15
影响因子：
2.9
通讯作者：
Cohenford M
中科院分区：
文献类型：
--
作者：
Frost L;Chaudhry M;Bell T;Cohenford M
糖对蛋白质的翻译后修饰已在糖尿病和经典半乳糖血症中得到证实。在糖尿病中，糖化过程是由于D-葡萄糖与蛋白质（如白蛋白和血红蛋白）发生非酶反应而发生的，这些蛋白质目前被用作监测糖尿病治疗期间饮食控制和治疗效率的重要工具。在典型的半乳糖血症中，D-半乳糖也有助于糖化蛋白的形成，这表明类似于葡萄糖化蛋白的糖尿病，半乳糖化蛋白的监测可能有助于半乳糖血症患者的管理。该项目的目标是：1）半乳糖化HSA，在体外; 2）确定，通过硼氢化钠依赖的质量肽作图方法，在HSA的半乳糖化位点;和3）比较HSA的半乳糖化位点与蛋白质的报告的葡萄糖化位点。用硼氢化钠处理半乳糖化的HSA稳定了蛋白质上的缩合糖，并通过串联MS产生了离散的碎片模式，从而可以可靠地鉴定HSA的半乳糖化位点。LC/ESI/MS结合串联MS，揭示了HSA中乳糖化的主要位点是赖氨酸12，233，281/276，414和525的氨基。赖氨酰残基12，233，276，和525先前被报道为D-葡萄糖与HSA的非酶结合的特权位点。
The post-translational modification of proteins by sugars has been demonstrated in diabetes and classical galactosemia. In diabetes, the glycation process occurs as a result of D-glucose nonenzymatically reacting with proteins such as albumin and hemoglobin, used today as important tools to monitor the efficiency of dietary control and therapy during treatment of diabetes. In classical galactosemia, D-galactose contributes to the formation of glycated proteins also, suggesting that akin to diabetes with glucated proteins, the monitoring of galactated proteins may facilitate management of patients with galactosemia. The objectives of this project were: 1) galactate HSA, in vitro; 2) determine, by a sodium borohydride dependent mass peptide mapping method, the galactation sites in HSA; and 3) compare HSA’s galactation sites with the protein’s reported glucation sites. Treatment of galactated HSA with sodium borohydride stabilized the condensed sugars on the protein and yielded discrete fragmentation patterns by tandem MS, allowing reliable identification of HSA’s galactation sites. LC/ESI/MS, in combination with tandem MS, revealed the principal sites of galactation in HSA were the amino groups of lysine 12, 233, 281/276, 414 and 525. Lysyl residues 12, 233, 276, and 525 were previously reported as privileged sites for the nonenzymatic binding of D-glucose with HSA.
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影响因子：
4.8
作者：
Howard, MJ;Smales, CM
通讯作者：
Smales, CM
影响因子：
3.3
作者：
Kislinger, T;Humeny, A;Pischetsrieder, M
通讯作者：
Pischetsrieder, M
DOI：
10.1111/j.1651-2227.1981.tb05770.x
发表时间：
1981-01-01
期刊：
ACTA PAEDIATRICA SCANDINAVICA
影响因子：
--
作者：
HOWARD, NJ;MONAGHAN, H;MARTIN, JM
通讯作者：
MARTIN, JM
影响因子：
8
作者：
Bosch, AM;Grootenhuis, MA;Last, BF
通讯作者：
Last, BF
影响因子：
1.7
作者：
Irene Ledesma-Osuna, Ana;Ramos-Clamont, Gabriela;Vazquez-Moreno, Luz
通讯作者：
Vazquez-Moreno, Luz