The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas
The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas
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DOI：
10.1016/s0967-5868(03)00072-9
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发表时间：
2003-07-01
影响因子：
2
通讯作者：
Frysak, Z
中科院分区：
文献类型：
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作者：
Cap, J;Cerman, J;Frysak, Z
为了探讨生长激素分泌腺瘤的组织病理学变化和增殖及凋亡活性，我们比较了14例术前用生长抑素类似物预处理的病例和17例未预处理的病例。除常规组织学、免疫细胞化学检测所有垂体激素、半胱天冬酶-3、细胞角蛋白-18和“M30抗原”外，还进行了其凋亡特异性片段的检测。肿瘤的增殖活性通过Ki-67抗原表达来确定。在治疗的腺瘤更显着的退行性变化，发现伴随着血管周围纤维化的代偿性增加。治疗组Ki-67标记指数（平均2.5，中位数1.6/mil）低于未治疗组（平均9.4，中位数5.0/mil）。差异具有统计学意义（使用曼-惠特尼秩和检验，p = 0.049）。在14个预处理的腺瘤中，仅在2个中检测到细胞凋亡，并且在未处理组中更频繁（9/17）且更显著。(C)2003爱思唯尔科技有限公司版权所有。
With the aim to investigate histopathological changes and proliferative and apoptotic activity in GH-secreting adenomas we compared 14 cases pre-treated with somatostatin analogues before surgery with a reference group of 17 un-pretreated ones. Besides routine histology, immunocytochemical detection of all pituitary hormones, caspase-3, cytokeratin-18, and "M30 antigen", its apoptosis-specific fragment was performed. Proliferation activity of the tumour was determined by the Ki-67 antigen expression. In treated adenomas more prominent regressive changes were found accompanied by compensatory increase in perivascular fibrosis. The Ki-67 labelling index was lower in treated group (mean 2.5, median 1.6 per mille) than in untreated patients (mean 9.4, median 5.0 per mille). The difference was statistically significant (p = 0.049 using Mann-Whitney Rank Sum Test). Apoptosis was detected in only 2 of the 14 pre-treated adenomas, and it was more frequent (9/17) and more prominent in the untreated group. (C) 2003 Elsevier Science Ltd. All rights reserved.