Two-photon absorption properties of fluorescent proteins.
Two-photon absorption properties of fluorescent proteins.
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DOI：
10.1038/nmeth.1596
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发表时间：
2011-05
期刊：
影响因子：
48
通讯作者：
Rebane A
中科院分区：
文献类型：
--
作者：
Drobizhev M;Makarov NS;Tillo SE;Hughes TE;Rebane A
荧光蛋白的双光子激发是一种有吸引力的成像活体系统的方法。今天，研究人员渴望知道哪些蛋白质是最亮的，以及最佳激发波长是什么。在这里，我们回顾了各种荧光蛋白的双光子吸收特性，包括新的远红外变体，以产生一个全面的指导选择正确的FP和激发波长的双光子应用。
Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest, and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right FP and excitation wavelength for two-photon applications.