Impaired masking responses to light in melanopsin-knockout mice
Impaired masking responses to light in melanopsin-knockout mice
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DOI：
10.1081/cbi-120026043
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Hattar, S
中科院分区：
文献类型：
--
作者：
Mrosovsky, N;Hattar, S
有两种方法可以使动物将其行为限制在夜间或白天的小生境中。一种是同步内源性时钟，进而控制睡眠-觉醒周期。另一种是直接对光照做出反应，并产生活动变化。在小鼠中，高照明水平抑制运动（负掩蔽），低照明水平增强运动（正掩蔽）。为了研究新发现的视蛋白样蛋白黑视蛋白在掩蔽中的作用，我们使用了夜间给予的1小时和3小时脉冲光，以及3.5：3.5小时的光暗（LD）周期。缺乏黑视素基因的小鼠在昏暗的灯光下运动能力正常增强，但在明亮的灯光下运动能力受到抑制。这种损害仅在10勒克斯或更亮的光下才明显。这表明视网膜神经节细胞中的黑视素参与掩蔽，就像它参与瞳孔收缩和相移一样。黑视素在长时间维持掩蔽反应中特别重要。
There are two ways in which an animal can confine its behavior to a nocturnal or diurnal niche. One is to synchronize an endogenous clock that in turn controls the sleep-wake cycle. The other is to respond directly to illumination with changes in activity. In mice, high illumination levels suppress locomotion (negative masking) and low illumination levels enhance locomotion (positive masking). To investigate the role of the newly discovered opsin-like protein melanopsin in masking, we used 1h and 3h pulses of light-given in the night, and also a 3.5 : 3.5h light dark (LD) cycle. Mice lacking the melanopsin gene had normal enhancement of locomotion in the presence of dim lights but an impaired suppression of locomotion in the presence of bright light. This impairment was evident only with lights in the order of 10 lux or brighter. This suggests that melanopsin in retinal ganglion cells is involved in masking, as it is in pupil contraction and phase shifts. Melanopsin is especially important in maintaining masking responses over long periods.