Analysis of antioxidants by microchip capillary electrophoresis with chemiluminescence detection based on luminol reaction
Analysis of antioxidants by microchip capillary electrophoresis with chemiluminescence detection based on luminol reaction
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DOI：
10.1016/j.talanta.2008.03.026
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发表时间：
2008-12
期刊：
影响因子：
6.1
通讯作者：
K. Tsukagoshi;Takahiro Saito;R. Nakajima
中科院分区：
文献类型：
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作者：
K. Tsukagoshi;Takahiro Saito;R. Nakajima
设计了一种包含两个用于电泳流的微通道和一个用于泵送流的微流线的微芯片，用于通过微芯片毛细管电泳和化学发光检测系统来分析抗氧化剂。该体系采用鲁米诺与过氧化氢在Cu(II)催化剂存在下发生化学发光反应，产生超氧自由基阴离子等活性氧。使用该系统将硝基蓝四唑、超氧化物歧化酶和儿茶素（来自绿茶的儿茶素混合物）作为抗氧化剂的模型分析物进行分析。根据活性氧（超氧自由基阴离子）和抗氧化剂（分析物）之间的反应，观察到由化学发光反应形成的相对于基线的负峰。分析物混合物的成分硝基蓝四唑和超氧化物歧化酶在大约 10 天内得到了很好的分离和检测。 2分钟2 分钟左右还检测到了儿茶素，导致响应曲线在 0.1-1mM 范围内。成功测定了市售绿茶饮料实际样品中儿茶素的含量。
A microchip including two micro-channels for electrophoretic flow and one micro-flow line for pumping flow was designed for the analysis of antioxidants by microchip capillary electrophoresis with a chemiluminescence detection system. The chemiluminescence reaction of luminol with hydrogen peroxide in the presence of Cu(II) catalyst was adopted in the present system, where active oxygen species, such as superoxide radical anions, generates. Nitroblue tetrazolium, superoxide dismutase, and catechin (catechin mixture from green tea) were analyzed as model analytes of antioxidants using the system. Negative peaks from baseline formed by the chemiluminescence reaction were observed based on reaction between active oxygen (superoxide radical anion) and antioxidants (analytes). The components of analytes mixture, nitroblue tetrazolium and superoxide dismutase, were well separated and detected within ca. 2min. Catechin was also detected around 2min, resulting in a response curve over the range of 0.1–1mM. The amounts of catechin in the real samples of commercial green tea beverages were successfully determined.