rtfA controls development, secondary metabolism, and virulence in Aspergillus fumigatus.
rtfA controls development, secondary metabolism, and virulence in Aspergillus fumigatus.
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DOI：
10.1371/journal.pone.0176702
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Calvo AM
中科院分区：
文献类型：
--
作者：
Myers RR;Smith TD;Elsawa SF;Puel O;Tadrist S;Calvo AM
由烟曲霉引起的侵袭性曲霉病是免疫功能低下患者感染相关死亡的主要原因。为了寻找潜在的遗传靶点来控制A.我们的特点rtfA，一个基因编码一个假定的RNA聚合酶II转录延伸因子样蛋白。我们最近的工作表明，在模式真菌构巢曲霉中的rtfA直系同源物调节形态发生和次生代谢。本文综述了我国条件致病菌A.对烟曲霉rtfA基因的研究表明，该基因影响真菌生长和分生孢子形成，以及次级代谢物色喹缬氨酸F、假黄菌素A、烟喹唑啉C、festuclavine和烟曲霉素A、B和C的产生。此外，rtfA影响蛋白酶活性水平、对氧化应激的敏感性和粘附能力，所有这些因素在致病性中都是重要的。此外，rtfA被证明是不可缺少的正常毒力使用大蜡螟以及鼠感染模型系统。
Invasive aspergillosis by Aspergillus fumigatus is a leading cause of infection-related mortality in immune-compromised patients. In order to discover potential genetic targets to control A. fumigatus infections we characterized rtfA, a gene encoding a putative RNA polymerase II transcription elongation factor-like protein. Our recent work has shown that the rtfA ortholog in the model fungus Aspergillus nidulans regulates morphogenesis and secondary metabolism. The present study on the opportunistic pathogen A. fumigatus rtfA gene revealed that this gene influences fungal growth and conidiation, as well as production of the secondary metabolites tryptoquivaline F, pseurotin A, fumiquinazoline C, festuclavine, and fumigaclavines A, B and C. Additionally, rtfA influences protease activity levels, the sensitivity to oxidative stress and adhesion capacity, all factors important in pathogenicity. Furthermore, rtfA was shown to be indispensable for normal virulence using Galleria mellonella as well as murine infection model systems.