Effects of Extended Freezer Storage on the Integrity of Human Milk
Effects of Extended Freezer Storage on the Integrity of Human Milk
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DOI：
10.1016/j.jpeds.2016.06.024
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发表时间：
2016-10-01
影响因子：
5.1
通讯作者：
Schanler, Richard J.
中科院分区：
文献类型：
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作者：
Ahrabi, Ali Faraghi;Handa, Deepali;Schanler, Richard J.
目的检测新鲜母乳和预先冷藏的母乳在长期冷藏条件下的完整性(pH值、细菌总数、宿主防御因子、营养成分和渗透压)。样品分为基线，在-20℃(新鲜冷冻)下储存1、3、6和9个月，以及在-20℃储存1-9个月之前在+4℃储存72小时(冷藏冷冻)。结果冷藏后牛奶的pH值、细菌总数、革兰氏阳性和革兰氏阴性菌落数、总蛋白质、脂肪、非酯化脂肪酸、乳铁蛋白、分泌型IgA和渗透压均显著下降(P
Objective To examine the integrity (pH, bacterial counts, host defense factors, nutrient contents, and osmolality) of freshly expressed and previously refrigerated human milk subjected to long-term freezer storage.Study design Mothers donated 100 mL of freshly expressed milk. Samples were divided into baseline, storage at -20 degrees C (fresh frozen) for 1, 3, 6, and 9 months, and prior storage at + 4 degrees C for 72 hours (refrigerated frozen) before storage at-20 degrees C for 1 to 9 months. Samples were analyzed for pH, total bacterial colony count, gram-positive and gram-negative colony counts, and concentrations of total protein, fat, nonesterified fatty acids, lactoferrin, secretory IgA, and osmolality.Results Milk pH, total bacterial colony count, and Gram-positive colony counts decreased significantly with freezer storage (P