Control of cell adhesion and proliferation utilizing polysaccharide composite film scaffolds
Control of cell adhesion and proliferation utilizing polysaccharide composite film scaffolds
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DOI：
10.1016/j.colsurfb.2017.09.025
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发表时间：
2017-12-01
影响因子：
5.8
通讯作者：
Hashizume, Mineo
中科院分区：
文献类型：
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作者：
Iijima, Kazutoshi;Tsuji, Yuna;Hashizume, Mineo
我们利用热压技术制备了阴离子多糖与壳聚糖(CHI)的复合膜。为了验证这些膜作为细胞支架的通用性，本研究考察了不同种类的阴离子多糖与蛋白质修饰的复合膜上成纤维细胞的黏附和增殖情况。细胞在肝素/CHI和海藻酸/CHI膜上生长良好，而在硫酸软骨素C(CS)/CHI和透明质酸/CHI膜上生长良好。细胞黏附和增殖行为的不同可以用阴离子多糖的生化功能和薄膜的物理性质如形态、储存模数、c-电位和溶胀率的不同来解释。其中，CS/CHI膜上的细胞数量几乎没有变化。用整合素刺激物、TNIIIA2多肽和血小板衍生生长因子B研究了CS/CHI膜生长抑制的机制。提示生长抑制是由于缺少纤维连接蛋白-整合素生长信号所致。用纤维连接蛋白对CS/CHI膜进行表面修饰，可促进细胞的黏附和增殖。这些结果表明，由阴离子多糖的化学物种或膜的表面修饰形成的多糖复合膜的化学和物理性能可以调节细胞在其上的黏附和增殖性能。(C)2017爱思唯尔B.V.保留所有权利。
We have developed polysaccharide composite films made of anionic polysaccharides and chitosan (CHI) by utilizing hot press techniques. In order to demonstrate the versatility of these films as cell scaffolds, the present study investigated the adhesion and proliferation of fibroblasts on composite films prepared by using various kinds of anionic polysaccharides and that were modified with proteins. Cells were spread on heparin/CHI and alginic acid/CHI films and grew well, whereas those on chondroitin sulfate C (CS)/CHI and hyaluronic acid/CHI films were round in shape. The differences in adhesion and proliferation behaviors of cells could be explained by the differences in the biochemical function of the anionic polysaccharides and the physical properties of the films such as morphology, storage modulus, c-potentials, and swelling ratios. Among them, the number of cells on CS/CHI films remained almost unchanged. The mechanisms underlying growth suppression on CS/CHI films were investigated by using an integrin stimulator, the TNIIIA2 peptide, and platelet-derived growth factor-B. It was indicated that the growth suppression was due to the lack of fibronectin-integrin growth signaling. The surface modification of CS/CHI films with fibronectin promoted the adhesion and proliferation of cells. These results show that the chemical and physical properties of the polysaccharide composite films, which resulted from the chemical species of anionic polysaccharides or surface modifications of the films, can modulate cell adhesion and proliferation properties thereon. (C) 2017 Elsevier B.V. All rights reserved.