Effects of Choto-san and hooks and stems of Uncaria sinensis on antioxidant enzyme activities in the gerbil brain after transient forebrain ischemia
Effects of Choto-san and hooks and stems of Uncaria sinensis on antioxidant enzyme activities in the gerbil brain after transient forebrain ischemia
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DOI：
10.1016/j.jep.2004.08.018
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Terasawa, K
中科院分区：
文献类型：
--
作者：
Yokoyama, K;Shimada, Y;Terasawa, K
以前，我们发现，口服给药的Choto-san，汉方，钩和茎的钩藤哈维兰（茜草科），药用植物，包括Choto-san，增强超氧阴离子和羟基自由基清除活性，在海马，并防止迟发性神经元死亡的海马CA 1区的锥体细胞在短暂的前脑缺血沙鼠模型。为了探讨内源性抗氧化酶是否参与了上述机制，我们采用相同的实验模型，研究了朝天椒提取物（CSE）和钩藤提取物（USE）对脑组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。将1.0%CSE或3.0%USE溶于水中，并从缺血/再灌注（i/rp）前7天起随意提供给沙鼠。连续7天给予CSE或USE而不进行i/rp程序可使海马和皮层CAT活性增强，但对SOD和GSH-Px活性无明显影响。CSE在i/rp后7 d和3 h分别升高海马和皮层中CAT活性。在i/rp后3 h和7 d，USE升高了海马和皮层的CAT活性。提示CSE和USE对短暂性脑缺血所致神经元损伤的保护作用机制之一可能是增强脑内CAT活性。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Previously, we revealed that oral administrations of Choto-san, a Kampo formula, and the hooks and stems of Uncaria sinensis Haviland (Rubiaceae), a medicinal plant comprising Choto-san, enhanced superoxide anion and hydroxyl radical scavenging activities in the hippocampus, and prevented delayed neuronal death of pyramidal cells in the hippocampal CA1 region in a transient forebrain ischemia gerbil model. In the present study, for the purpose of clarifying whether the endogenous antioxidant enzymes contribute to these mechanisms, we investigated the effects of Choto-san extract (CSE) and Uncaria sinensis extract (USE) on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in the brain by using the same experimental model. 1.0% CSE or 3.0% USE were dissolved in water and provided to gerbils ad libitum from 7 days prior to ischemia/reperfusion (i/rp). Seven days of continuous administrations of CSE or USE without i/rp procedure enhanced CAT activity but not SOD and GSH-Px activities in both the hippocampus and cortex. CSE elevated CAT activity in the hippocampus at 7 days and in the cortex at 3 h after i/rp. USE raised CAT activity in both the hippocampus and cortex at 3 h and 7 days after i/rp. These results suggest that one of the mechanisms of the protective effects of CSE and USE against transient brain ischemia-induced neuronal damage may be their enhancing effect on CAT activity in the brain. (C) 2004 Elsevier Ireland Ltd. All rights reserved.