The interleukin-1-induced increase of substance P in sympathetic ganglia is not mediated by ciliary neurotrophic factor.
The interleukin-1-induced increase of substance P in sympathetic ganglia is not mediated by ciliary neurotrophic factor.
复制标题
白细胞介素1诱导的交感神经节P物质的增加不是由睫状神经营养因子介导的。
DOI：
10.1002/jnr.490380606
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发表时间：
1994
影响因子：
4.2
通讯作者：
Jonakait,GM
中科院分区：
文献类型：
--
作者：
Ding,M;Hart,RP;Shadiack,AM;Jonakait,GM
白介素1(IL-1)对交感神经节P物质(SP)的诱导作用需要一种存在于IL-1条件培养液(IL-1 CM)中的可溶性中间分子。所需的中间体之一是白血病抑制因子(LIF；Shadiack等人，J Neurosci 13：2601-2609,1993)。在本研究中，我们探讨了睫状神经营养因子(CNTF)是交感SP诱导IL-1的另一个中介的可能性。CNTF通过提高SP和胆碱乙酰转移酶的活性来模拟IL-1 CM的作用-CNTF的特定中和抗血清可以阻断的作用。然而，IL-1 CM和CNTF对去极化药物的反应不同：KCl(40 MM)阻断IL-1 CM(和LIF)的作用，但增强CNTF的作用。此外，在IL-1 CM中未检测到CNTF生物活性和CNTF蛋白。中和CNTF抗血清不能阻断IL-1或IL-1 CM的作用，这表明可溶性或膜结合形式的CNTF分子在对IL-1作用的直接反应中都不活跃。虽然Northern杂交证实新生神经节中同时存在CNTF和CNTF受体mRNA，但培养和IL-1处理都不能改变这些mRNA水平。这些数据表明，虽然CNTF在交感神经节中存在并可能活跃，但它不是SP诱导IL-1的中介物质。
Interleukin-1 (IL-1) induction of substance P (SP) in cultured sympathetic ganglia requires a soluble intermediate molecule that is present in IL-1 conditioned medium (IL-1CM). One of the required intermediates is leukemia inhibitory factor (LIF; Shadiack et al., J Neurosci 13: 2601–2609, 1993). In the present study we have examined the possibility that ciliary neurotrophic factor (CNTF) is another intermediate involved in the IL-1 induction of sympathetic SP. CNTF mimics the action of IL-1CM by raising both SP and choline acetyltransferase activity---actions that are blocked by a specific neutralizing antiserum for CNTF. However, IL-1CM and CNTF differ in their response to depolarizing agents: while KCl (40 mM) blocks the action of IL-1CM (and LIF), it enhances the action of CNTF. Furthermore, neither CNTF bioactivity nor CNTF protein is detected in IL-1CM. Neutralizing antiserum to CNTF fails to block the action of either IL-1 or IL-1CM, suggesting that neither a soluble nor a membrane-bound form of the molecule is active in direct response to IL-1 action. While Northern blots confirm the presence of both CNTF and CNTF receptor mRNA in neonatal ganglia, neither culturing nor IL-1 treatment alters these mRNA levels. These data taken together suggest that while CNTF is present and possibly active in sympathetic ganglia, it is not a mediator of the IL-1 induction of SP.© 1994 Wiley-Liss, Inc.