Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles.
Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles.
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DOI：
10.1007/978-1-4939-2330-4_5
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Hamm, Heidi E
中科院分区：
文献类型：
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作者：
Kaya, Ali I;Iverson, T M;Hamm, Heidi E
视紫红质是G蛋白偶联受体（GPCR）的原型成员。这种光感受器负责在光激活后与其异源三聚体G蛋白转导素（Gt）相互作用时启动视觉信号转导级联。像所有跨膜蛋白一样，视紫红质嵌入磷脂双层内。许多研究已经提出，这种双层的膜组成是激活过程中受体功能的重要因素。在这里，我们描述的方法和测定用于评估的功能，纯化和重建的视紫红质在bicelles。
Rhodopsin is a prototypical member of the G protein-coupled receptors (GPCRs). This photoreceptor is responsible for initiating the visual signaling transduction cascade upon interaction with its heterotrimeric G protein, transducin (Gt), after light activation. Like all transmembrane proteins, rhodopsin is embedded within a phospholipid bilayer. Many studies have proposed that the membrane composition of this bilayer is an important factor for receptor function during the activation process. Here we describe the methods and assays used to evaluate the function of purified and reconstituted rhodopsin in bicelles.